ArticlePloS one2019
Effect of different forage-to-concentrate ratios on ruminal bacterial structure and real-time methane production in sheep.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Camelina straw as an eco-friendly forage alternative for finishing lambs: impacts on performance, rumen function and behavior.Veterinary and animal science · 2025Article
- Effects of Onion Peel Inclusion on In Vitro Fermentation, Methane and Carbon Dioxide Emissions, and Nutrient Degradability in Dairy Cow Diets.Animals : an open access journal from MDPI · 2025Article
- The effect of the forage-to-concentrate ratio of the total mixed ration on ruminal microbiota changes in Alpine dairy goats.Journal of animal science · 2025Article
- Effects of High-Concentrate Diets on Growth Performance, Serum Biochemical Indexes, and Rumen Microbiota in House-Fed Yaks.Animals : an open access journal from MDPI · 2024Article
- Impact of Seasonal Variation in Pasture on Rumen Microbial Community and Volatile Fatty Acids in Grazing Yaks: Insights from High-Altitude Environments.Microorganisms · 2024Article
- Impact of 5-20% Hydroponic Wheat Sprouts Inclusion on Growth and Metabolic Parameters of Growing Ewes.Animals : an open access journal from MDPI · 2024Article
- Evaluation of yeast-based additives on rumen fermentation in high- and low-concentrate diets using a dual-flow continuous culture system.Translational animal science · 2024Article
- Rumen microbiota helps Tibetan sheep obtain energy more efficiently to survive in the extreme environment of the Qinghai-Tibet Plateau.Frontiers in microbiology · 2024Article
- Methane prediction equations including genera of rumen bacteria as predictor variables improve prediction accuracy.Scientific reports · 2023Article
- Metagenomics analysis reveals differences in rumen microbiota in cows with low and high milk protein percentage.Applied microbiology and biotechnology · 2023Article
- Different microbial genera drive methane emissions in beef cattle fed with two extreme diets.Frontiers in microbiology · 2023Article
- Comparison of metabolites in rumen fluid, urine, and feces of dairy cow from subacute ruminal acidosis model measured by proton nuclear magnetic resonance spectroscopy.Animal bioscience · 2023Article
- The Effect of Indigo (Animals : an open access journal from MDPI · 2022Article
- Effects of Concentrate Supplementation on Growth Performance, Rumen Fermentation, and Bacterial Community Composition in Grazing Yaks during the Warm Season.Animals : an open access journal from MDPI · 2022Article
- Time-course alterations of gut microbiota and short-chain fatty acids after short-term lincomycin exposure in young swine.Applied microbiology and biotechnology · 2021Article
- The Effect of Forage-to-Concentrate Ratio onAnimals : an open access journal from MDPI · 2021Article
- Dose response of biochar and wood vinegar on in vitro batch culture ruminal fermentation using contrasting feed substrates.Translational animal science · 2021Article
- Holstein and Jersey Steers Differ in Rumen Microbiota and Enteric Methane Emissions Even Fed the Same Total Mixed Ration.Frontiers in microbiology · 2021Article
- Changes in Carbohydrate Composition in Fermented Total Mixed Ration and Its Effects onFrontiers in microbiology · 2021Article
- Presence of Adult Companion Goats Favors the Rumen Microbial and Functional Development in Artificially Reared Kids.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Emission from ruminants has become one of the largest sources of anthropogenic methane emission in China. The structure of the rumen flora has a significant effect on methane production. To establish a more accurate prediction model for methane production, the rumen flora should be one of the most important parameters. The objective of the present study was to investigate the relationship among changes in rumen flora, nutrient levels, and methane production in sheep fed with the diets of different forage-to-concentration ratios, as well as to screen for significantly different dominant genera. Nine rumen-cannulated hybrid sheep were separated into three groups and fed three diets with forage-to-concentration ratios of 50:50, 70:30, and 90:10. Three proportions of the diets were fed according to a 3 × 3 incomplete Latin square, design during three periods of 15d each. The ruminal fluid was collected for real-time polymerase chain reaction (real-time PCR), high-throughput sequencing and in vitro rumen fermentation in a new real-time fermentation system wit. Twenty-two genera were screened, the abundance of which varied linearly with forage-to-concentration ratios and methane production. In addition, during the 12-hour in vitro fermentation, the appearance of peak concentration was delayed by 26-27min with the different structure of rumen bacteria. The fiber-degrading bacteria were positively correlated with this phenomenon, but starch-degrading and protein-degrading bacteria were negative correlated. These results would facilitate macro-control of rumen microorganisms and better management of diets for improved nutrition in ruminants. In addition, our findings would help in screening bacterial genera that are highly correlated with methane production.
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