Evidence map›Paper›PMID 31116195›Full record

ArticleThe Analyst2019

Inkjet-printed micro-calibration standards for ultraquantitative Raman spectral cytometry.

Vernon LaLone, Maria V Fawaz, Jomar Morales-Mercado, Márcio A Mourão, Catherine S Snyder, Sang Yeop Kim, Andrew P Lieberman, Anish Tuteja, Geeta Mehta, Theodore J Standiford and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Analyst, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Vernon LaLoneDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.ORCID http://orcid.org/0000-0001-6043-8993
Maria V FawazDepartment of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0002-4743-1444
Jomar Morales-MercadoSchool of Sciences, Technology, and Environment, Universidad Ana G. Méndez Cupey Campus, San Juan, Puerto Rico.ORCID http://orcid.org/0000-0003-1090-0929
Márcio A MourãoCSCAR Center, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0003-0969-0266
Catherine S SnyderDepartment of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0002-6636-2798
Sang Yeop KimDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.ORCID http://orcid.org/0000-0002-5781-9039
Andrew P LiebermanDepartment of Pathology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
Anish TutejaDepartment of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA and Biointerfaces Institute, Macromolecular Science and Engineering, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0002-2383-4572
Geeta MehtaDepartment of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA and Department of Biomedical Engineering, Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0001-5967-6425
Theodore J StandifordDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0002-5890-4470
Krishnan RaghavendranDepartment of Surgery, Michigan Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0002-1405-8356
Kerby SheddenCSCAR Center, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0001-7222-6664
Anna SchwendemanDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.ORCID http://orcid.org/0000-0002-8023-8080
Kathleen A StringerDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA and Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.ORCID http://orcid.org/0000-0003-0238-7774
Gus R RosaniaDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.ORCID http://orcid.org/0000-0003-3214-5565

Funding

INTERDEPARTMENTAL TRAINING IN PHARMACOLOGICAL SCIENCEST32GM007767 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ISOM, LORI L. · 1985 to 2019
$10.2M
Unraveling mechanisms of Niemann-Pick C neuropathology with mouse modelsR01NS063967 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LIEBERMAN, ANDREW P · 2009 to 2020
$4.4M
Chemical Address Tags: A Cheminformatics & Image Data Management and Analysis Plan - SupplementR01GM078200 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ROSANIA, GUS R · 2006 to 2015
$4.2M
L-Carnitine As A Metabolic Biomarker of Drug Toxicity RiskR01GM127787 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ROSANIA, GUS R · 2019 to 2022
$2.0M
Training Program in Translational Cardiovascular Research and EntrepreneurshipT32HL125242 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MICHELE, DANIEL E · 2015 to 2024
$1.9M
L-Carnitine Pharmacometabolomics in Sepsis (CaPS)R01GM111400 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI STRINGER, KATHLEEN A · 2016 to 2019
$1.2M
Optimization of synthetic HDL nanoparticles for treatment Niemann-Pick Type CR21NS111191 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SCHWENDEMAN, ANNA · 2019 to 2019
$429k
NHLBI NIH HHS T32 HL125242NIGMS NIH HHS R01 GM078200NIGMS NIH HHS R01 GM111400NIGMS NIH HHS R01 GM127787NIGMS NIH HHS T32 GM007767NINDS NIH HHS R01 NS063967NINDS NIH HHS R21 NS111191
6 · The paper itself

Abstract

Herein we report the development of a cytometric analysis platform for measuring the contents of individual cells in absolute (picogram) scales; this study represents the first report of Raman-based quantitation of the absolute mass - or the total amount - of multiple endogenous biomolecules within single-cells. To enable ultraquantitative calibration, we engineered single-cell-sized micro-calibration standards of known composition by inkjet-printer deposition of biomolecular components in microarrays across the surface of silicon chips. We demonstrate clinical feasibility by characterizing the compositional phenotype of human skin fibroblast and porcine alveolar macrophage cell populations in the respective contexts of Niemann-Pick disease and drug-induced phospholipidosis: two types of lipid storage disorders. We envision this microanalytical platform as the foundation for many future biomedical applications, ranging from diagnostic assays to pathological analysis to advanced pharmaco/toxicokinetic research studies.

Identifiers

PMID31116195
PMCPMC6711383

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.