Evidence map›Paper›PMID 31115772›Full record

ArticleMikrochimica acta2019

Distinguishing cancer cell lines at a single living cell level via detection of sialic acid by dual-channel plasmonic imaging and by using a SERS-microfluidic droplet platform.

Lili Cong, Lijia Liang, Fanghao Cao, Dan Sun, Jing Yue, Weiqing Xu, Chongyang Liang, Shuping Xu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Lili CongState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China.
Lijia LiangState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China.
Fanghao CaoState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China.
Dan SunState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China.
Jing YueState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China.
Weiqing XuState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China.
Chongyang LiangInstitute of Frontier Medical Science, Jilin University, Changchun, 130021, People's Republic of China.
Shuping XuState Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Ave., Changchun, 130012, People's Republic of China. xusp@jlu.edu.cn.ORCID 0000-0002-6216-6175
Jilin University · CN

Funding

National Natural Science Foundation of China 21573087National Natural Science Foundation of China 21573092National Natural Science Foundation of China 21873039
6 · The paper itself

Abstract

A high-throughput, dual-channel single cell analytical method is described for the detection of sialic acid (SA) on single cell based on the use of microfluidic droplets integrated with plasmonic imaging and surface-enhanced Raman spectroscopy (SERS) with the assistance of a multifunctional metal nanoparticle-based probe. The multifunctional plasmonic nanoprobe was prepared by modifying silver nanoparticles (AgNPs) with 4-mercaptophenylboronic acid (MPBA) that both warrants SA recognition and acts as a Raman reporter. This nanoprobe is a high-contrast indicator under bright field imaging due to the strong energy loss feature of AgNPs, and also owns possesses a strong SERS enhancement capability toward MPBA. Cells incubated with the plasmonic nanoprobes were isolated in water-in-oil droplets and then were re-dispersed in a chamber array chip. High-precision profiles of SA on a single cell in one droplet were obtained by the bright field imaging and image processing. The SA expression levels on different cell lines (MCF-7, HepG2, SGC and BNL.CL2) traced by SERS spectroscopy were compared. The statistical data among different cell lines confirm that the SA expression levels on cancer cells are much higher than that on normal cells. Single cell analysis further revealed that the cell-to-cell variations are more obvious in cancer cell lines. This study provides a valuable tool for understanding glycan-related biochemical processes. Graphical abstract A high-throughput, dual-channel microfluidic droplet platform succeeded in distinguishing different cancer cell lines at single living cell level integrated with plasmonic imaging and surface-enhanced Raman spectroscopy with assistance of a multifunctional metal nanoparticle-based probe.

Indexed as

AnimalsBiomarkers, TumorBoronic AcidsCell Line, TumorHumansLab-On-A-Chip DevicesMetal NanoparticlesMiceMicrofluidic Analytical TechniquesN-Acetylneuraminic AcidNeoplasmsSilverSingle-Cell AnalysisSpectrum Analysis, RamanSulfhydryl Compounds4-mercaptophenylboronic acidBiomarkers, TumorBoronic AcidsN-Acetylneuraminic AcidSilverSulfhydryl Compounds4-Mercaptophenylboronic acidMicrofluidic dropletPlasmonic imagingSialic acidSilver nanoparticleSingle living cellSurface-enhanced Raman spectroscopy

Identifiers

PMID31115772
OpenAlexW2945698303

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.