ArticleMolecular medicine reports2019
Identification and integrative analysis of microRNAs and mRNAs involved in proliferation and invasion of pressure‑treated human liver cancer cell lines.
Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- High interstitial fluid pressure enhances USP1-dependent KIF11 protein stability to promote hepatocellular carcinoma progression.Journal of translational medicine · 2025Article
- Biomechanics in liver regeneration after partial hepatectomy.Frontiers in bioengineering and biotechnology · 2023Review
- Overexpression of pressure-responsive miRNA-5703 inhibits pressure-induced growth and metastasis of liver cancer.Journal of Cancer · 2022Article
- Protective effect of propofol via the regulation of ovarian granulosa cell proliferation and apoptosis.Experimental and therapeutic medicine · 2021Article
- MicroRNAs Regulating Hippo-YAP Signaling in Liver Cancer.Biomedicines · 2021Review
- Role of lncRNA PART1 in intervertebral disc degeneration and associated underlying mechanism.Experimental and therapeutic medicine · 2021Article
- Screening of differentially expressed miRNAs in tensile strain‑treated HepG2 cells by miRNA microarray analysis.Molecular medicine reports · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical pressure may contribute to the development of cancer; however, there is currently no evidence regarding the effect of mechanical pressure on liver cancer. In the present study, 2‑ and 3‑dimensional pressure‑loading systems were used to exert pressure on HepG2 and Huh‑7 cell lines. Cell proliferation and flow cytometry analyses were undertaken to observe the proliferative ability of pressure‑loaded cells. In addition, Transwell, wound‑healing and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assays were applied to evaluate the migratory and invasive ability of pressurized cells. Analyses of microRNA (miRNA) and mRNA expression profiles were performed to screen for differentially expressed miRNAs and mRNAs, which were validated by RT‑qPCR. Bioinformatics analyses were subsequently performed to investigate the putative target genes and associated pathways. The proliferation and invasion of HepG2 and Huh‑7 cell lines were significantly increased under a pressure of 15 mmHg for 24 h. Under this condition, five differentially expressed miRNAs (fold change ≥1.2, P≤0.05) and 10,150 differentially expressed mRNAs (fold change ≥2, P≤0.05) were identified. A total of 1,309 genes were identified from the integrative analysis of miRNAs and mRNAs. In addition, the bioinformatics analyses revealed that the majority of these miRNAs and mRNAs were associated with several pathways associated with cell proliferation and invasion, including 'PI3K/Akt signaling pathway', 'focal adhesion', 'integrin‑mediated signaling pathway', 'FOXO signaling pathway' and 'Hippo signaling pathway'. The present study described the pressure‑dependent proliferation and invasion of liver cancer cells, and revealed the potential molecular mechanisms underlying them. The identification of miRNAs and their putative targets may also result in novel treatment strategies for liver cancer.
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