ArticleNucleic acids research2019
Eukaryotic initiation factor (eIF) 3 mediates Barley Yellow Dwarf Viral mRNA 3'-5' UTR interactions and 40S ribosomal subunit binding to facilitate cap-independent translation.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Retinoic acid is a translation inhibitor against chikungunya virus both in vitro and invivo.Virologica Sinica · 2026Article
- Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA Synthesis.International journal of molecular sciences · 2026Article
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- Long-distance RNA-RNA interactions shape dynamic RNA architectures and functional states in plant positive-strand RNA viruses.Frontiers in microbiology · 2026Review
- DDX3 Regulates the Cap-Independent Translation of the Japanese Encephalitis Virus via Its Interactions with PABP1 and the Untranslated Regions of the Viral Genome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Machine Learning to Identify Critical Biomarker Profiles in New SARS-CoV-2 Variants.Microorganisms · 2024Article
- Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana.Molecular plant pathology · 2024Article
- Conserved Structure Associated with Different 3'CITEs Is Important for Translation of Umbraviruses.Viruses · 2023Article
- Critical Roles of METTL3 in Translation Regulation of Cancer.Biomolecules · 2023Review
- Eukaryotic initiation factor 4F promotes a reorientation of eukaryotic initiation factor 3 binding on the 5' and the 3' UTRs of barley yellow dwarf virus mRNA.Nucleic acids research · 2022Article
- Effects of NFrontiers in oncology · 2022Review
- Translation of Plant RNA Viruses.Viruses · 2021Review
- Research progress concerning mOncology letters · 2021Review
- Enhancing Capsid Proteins Capacity in Plant Virus-Vector Interactions and Virus Transmission.Cells · 2021Review
- eIF3 and Its mRNA-Entry-Channel Arm Contribute to the Recruitment of mRNAs With Long 5'-Untranslated Regions.Frontiers in molecular biosciences · 2021Article
- The RNA of Maize Chlorotic Mottle Virus, an Obligatory Component of Maize Lethal Necrosis Disease, Is Translated via a Variant Panicum Mosaic Virus-Like Cap-Independent Translation Element.Journal of virology · 2020Article
- Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barley Yellow Dwarf Virus (BYDV) is a positive strand RNA virus that lacks the canonical 5' 7-methylguanosine cap and a 3' poly-A tail. Instead, BYDV utilizes a cruciform cap independent translation element (CITE) in its 3'UTR RNA (BYDV-like CITE or BTE) that binds eukaryotic translation initiation factor (eIF) 4F and recruits 40S ribosomal subunits in the presence of active helicase factors (eIF4A, eIF4B, eIF4F and ATP). A long-range, 5-nucleotide, base-pairing kissing loop interaction between the 3'BTE and a 5'UTR stem-loop is necessary for translation to initiate. The 40S-eIF complex does not bind to the BYDV 5'UTR, suggesting the involvement of additional factors. We identified eIF3 as a component of the 3'BTE recruited complex using affinity-tagged 3'BTE RNA pull-down assays. Fluorescence anisotropy binding and gel shift assays showed that the 3'BTE and 5'UTR RNAs can simultaneously and non-competitively bind eIF3 in the presence of active helicase factors forming a single, macromolecular complex. Further, quantitative studies showed eIF3 increased recruitment of the 40S subunit by more than 25-fold. We propose a new role for eIF3, where eIF3 bridges BYDV's UTRs, stabilizes the long-range 5'-3' interaction, and facilitates recruitment of the 40S-eIF complex to the 5'UTR, leading to translation initiation.
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