Evidence map›Paper›PMID 31114448›Full record

ArticleCancer cell international2019

Overexpression of SNHG12 regulates the viability and invasion of renal cell carcinoma cells through modulation of HIF1α.

Qiguang Chen, Wei Zhou, Shu-Qi Du, Da-Xin Gong, Jun Li, Jian-Bin Bi, Zhen-Hua Li, Zhe Zhang, Ze-Liang Li, Xian-Kui Liu and 1 more

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In one paragraph

Article in Cancer cell international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  11. Nucleolar Proteins and Non-Coding RNAs: Roles in Renal Cancer.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Qiguang Chen1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Wei Zhou2Department of Diagnostic Radiology, General Hospital of Shenyang Military Region, No.83, Wenhua Road, Shenyang, 110016 Liaoning People's Republic of China.
Shu-Qi Du1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Da-Xin Gong1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Jun Li1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Jian-Bin Bi1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Zhen-Hua Li1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Zhe Zhang1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Ze-Liang Li1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Xian-Kui Liu1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.
Chui-Ze Kong1Department of Urology, The First Affiliated Hospital of China Medical University, No.155 Nanjing Street, Shenyang, 110001 Liaoning People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCumulative evidences demonstrated the aberrant overexpression of Small Nucleolar RNA Host Gene 12 (SNHG12) in diverse human cancer. However, the expression status and involvement of SNHG12 in renal cell carcinoma is still elusive.

methodsThe expression of SNHG12 was determined by q-PCR. The transcriptional regulation was interrogated by luciferase reporter assay. Cell viability was measured with CCK-8 kit. The anchorage-independent was evaluated by soft agar assay. Cell apoptosis was analyzed by Annexin V/7-AAD double staining. The migration and invasion were determined by trans-well assay and wound scratch closure. The in vivo tumor growth was monitored in xenograft mice model. Protein expression was quantified by immunoblotting.

resultsSNHG12 was aberrantly up-regulated in renal carcinoma both in vivo and in vitro. High expression of SNHG12 associated with poor prognosis. Deficiency of SNHG12 significantly suppressed cell viability, anchorage-independent growth and induced apoptosis. In addition, SNHG12 silencing inhibited migrative and invasive in vitro and xenograft tumor growth in vivo. Mechanistically, SNHG12 modulated HIF1α expression via competing with miR-199a-5p, which consequently contributed to its oncogenic potential. MiR-199a-5p inhibition severely compromised SNHG12 silencing-elicited tumor repressive effects.

conclusionOur data uncovered a crucial role of SNHG12-miR-199a-5p-HIF1α axis in human renal cancer.

Indexed as

HIF1αLong non-coding RNAMiR-199a-5pRenal cancerSNHG12

Identifiers

PMID31114448
PMCPMC6518781

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