ArticleScientific reports2019
Aptasensor for multiplex detection of antibiotics based on FRET strategy combined with aptamer/graphene oxide complex.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Aptamer-Based Fluorescent Biosensors for Kanamycin Detection in Food Systems: Design Strategies, Sensing Mechanisms, and Practical Applications.Foods (Basel, Switzerland) · 2026Review
- Graphene-Based Biosensors: Enabling the Next Generation of Diagnostic Technologies-A Review.Biosensors · 2025Review
- Ampicillin detection using absorbance biosensors utilizing Mn-doped ZnS capped with chitosan micromaterials.Heliyon · 2024Article
- Interplay of graphene-DNA interactions: Unveiling sensing potential of graphene materials.Applied physics reviews · 2024Article
- Nanomaterial-based Electrochemical Sensors for Multiplex Medicinal Applications.Current topics in medicinal chemistry · 2024Review
- Recent Progress in Nanotechnology-Based Approaches for Food Monitoring.Nanomaterials (Basel, Switzerland) · 2022Review
- Electrochemical Carbamazepine Aptasensor for Therapeutic Drug Monitoring at the Point of Care.ACS omega · 2022Article
- Nanozyme-based sensors for detection of food biomarkers: a review.RSC advances · 2022Review
- The quantum dotChemical science · 2022Article
- Thiourea- and Amino-Substituted Benzoxadiazole Dyes with Large Stokes Shifts as Red-Emitting Probe Monomers for Imprinted Polymer Layers Targeting Carboxylate-Containing Antibiotics.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Graphene-Oxide-Based Fluoro- and Chromo-Genic Materials and Their Applications.Molecules (Basel, Switzerland) · 2022Review
- A Microfluidic Aptamer-Based Sensor for Detection of Mercury(II) and Lead(II) Ions in Water.Micromachines · 2021Article
- An Ultrasensitive Label-Free Fluorescent Aptasensor Platform for Detection of Sulfamethazine.International journal of nanomedicine · 2021Article
- Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay.Antibiotics (Basel, Switzerland) · 2020Article
- Graphene oxide and its derivatives as promising In-vitro bio-imaging platforms.Scientific reports · 2020Article
- Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs.Molecules (Basel, Switzerland) · 2020Review
- The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products.Biosensors · 2020Review
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Authors and funding
8 authors.
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Abstract
The development of a multiplexed sensing platform is necessary for highly selective, sensitive, and rapid screening of specific antibiotics. In this study, we designed a novel multiplex aptasensor for antibiotics by fluorescence resonance energy transfer (FRET) strategy using DNase I-assisted cyclic enzymatic signal amplification (CESA) method combined with aptamer/graphene oxide complex. The aptamers specific for sulfadimethoxine, kanamycin, and ampicillin were conjugated with Cyanine 3 (Cy3), 6-Carboxyfluorescein (FAM), and Cyanine 5 (Cy5), respectively, and graphene oxide (GO) was adopted to quench the fluorescence of the three different fluorophores with the efficiencies of 94.36%, 93.94%, and 96.97% for Cy3, FAM, and Cy5, respectively. CESA method was used for sensitive detection, resulting in a 2.1-fold increased signal compared to those of unamplified method. The aptasensor rapidly detected antibiotics in solution with limit of detection of 1.997, 2.664, and 2.337 ng/mL for sulfadimethoxine, kanamycin, and ampicillin, respectively. In addition, antibiotics dissolved in milk were efficiently detected with similar sensitivities. Multiplexed detection test proved that the fluorescently modified aptamers could work separately from each other. The results indicate that the aptasensor offers high specificity for each antibiotic and enables simultaneous and multicolor sensing for rapid screening of multiple antibiotics at the same time.
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