Evidence map›Paper›PMID 31113397›Full record

ArticleBMC nephrology2019

Transcriptome analysis of signaling pathways of human peritoneal mesothelial cells in response to different osmotic agents in a peritoneal dialysis solution.

Bin Liu, Shijian Feng, Ghida Dairi, Qiunong Guan, Irina Chafeeva, Hao Wang, Richard Liggins, Gerald da Roza, Jayachandran N Kizhakkedathu, Caigan Du

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC nephrology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 2 pooled it
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 syntheses or guidelines pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Bin LiuDepartment of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
Shijian FengDepartment of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
Ghida DairiDepartment of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
Qiunong GuanDepartment of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
Irina ChafeevaCentre for Blood Research, and Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Hao WangGeneral Hospital of Tianjin Medical University, No.154 Anshan Road, Heping District, Tianjin, 300052, China.
Richard LigginsCentre for Drug Research and Development, Vancouver, BC, Canada.
Gerald da RozaDivision of Nephrology, Department of Medicine, University of British Columbia, Vancouver, BC, Canada.
Jayachandran N KizhakkedathuCentre for Blood Research, and Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Caigan DuDepartment of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada. caigan@mail.ubc.ca.
University of British Columbia · CACentre for Drug Research and Development · CATianjin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGlucose is a primary osmotic agent in peritoneal dialysis (PD) solutions, but its long-term use causes structural alteration of the peritoneal membrane (PM). Hyperbranched polyglycerol (HPG) is a promising alternative to glucose. This study was designed to compare the cellular responses of human peritoneal mesothelial cells (HPMCs) to these two different osmotic agents in a hypertonic solution using transcriptome analysis.

methodsCultured HPMCs were repeatedly exposed to HPG-based or Physioneal 40 (PYS, glucose 2.27%) hypertonic solutions. Transcriptome datasets were produced using Agilent SurePrint G3 Human GE 8 × 60 microarray. Cellular signaling pathways were examined by Ingenuity Pathway Analysis (IPA). Protein expression was examined by flow cytometry analysis and Western blotting.

resultsThe HPG-containing solution was better tolerated compared with PYS, with less cell death and disruption of cell transcriptome. The levels of cell death in HPG- or PYS- exposed cells were positively correlated with the number of affected transcripts (HPG: 128 at day 3, 0 at day 7; PYS: 1799 at day 3, 212 at day 7). In addition to more affected "biosynthesis" and "cellular stress and death" pathways by PYS, both HPG and PYS commonly affected "sulfate biosynthesis", "unfolded protein response", "apoptosis signaling" and "NRF2-mediated oxidative stress response" pathways at day 3. PYS significantly up-regulated HLA-DMB and MMP12 in a time-dependent manner, and stimulated T cell adhesion to HPMCs.

conclusionThe lower cytotoxicity of hypertonic HPG solution is in agreement with its transient and minimal impact on the pathways for the "biosynthesis of cell constituents" and the "cellular stress and death". The significant up-regulation of HLA-DMB and MMP12 by PYS may be part of its initiation of immune response in the PM.

Indexed as

Cell Line, TransformedCell ProliferationCells, CulturedDialysis SolutionsDiuretics, OsmoticGene Expression ProfilingHumansJurkat CellsOrganic ChemicalsPeritoneal CavityPeritoneal DialysisPolymethacrylic AcidsSignal TransductionTranscriptomeDialysis SolutionsDiuretics, OsmoticHPAEG polymerOrganic ChemicalsPhysioneal 35Polymethacrylic AcidsGlucoseHyperbranched polyglycerolOsmotic agentsPeritoneal mesothelial cellsSignaling pathwaysTranscriptome

Identifiers

PMID31113397
PMCPMC6528310
OpenAlexW2947037524

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.