ReviewThe Journal of steroid biochemistry and molecular biology2019
Current strategies for quantification of estrogens in clinical research.
Review in The Journal of steroid biochemistry and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 93 citations in OpenAlex.
- Sensitive detection of unopposed estrogen using estrogen receptor functionalized nanoprobes for cancer diagnosis.Discover nano · 2026Review
- Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes.bioRxiv : the preprint server for biology · 2026Article
- Development of a Highly Sensitive Analytical System for Measuring 17β-Estradiol Using Fluorescent Molecular Probes.Sensors (Basel, Switzerland) · 2026Article
- Fetal Bovine Serum Modulates Primary Human Cell Phenotypes, Endothelial Barrier Function, Vasculogenesis, and Angiogenesis in a Sex-Specific Manner.Cellular and molecular bioengineering · 2025Article
- A Membrane-Based Strategy for the High-Throughput Determination of Steroidal Hormones in Human Urine Using Natural Deep Eutectic Solvents Combined With Liquid Chromatography Coupled With Diode Array Detector.Journal of separation science · 2025Article
- Mass spectrometric profiling of primary estrogens and estrogen metabolites in human stool and plasma partially elucidates the role of the gut microbiome in estrogen recycling.Molecular and cellular endocrinology · 2025Article
- Development of an accurate and sensitive assay for 2-methoxyestradiol using derivatization and liquid chromatography-tandem mass spectrometry.Practical laboratory medicine · 2025Article
- Triple-template surface imprinted magnetic polymers for wide-coverage extraction of steroid hormones from human serum.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Estrogen profiling in blood and brain: Effects of season and an aggressive interaction in a songbird.PloS one · 2025Article
- Mimicking women's endocrine milieu in mice for women's health-related studies.npj women's health · 2025Article
- Endogenous estrogen metabolites as oxidative stress mediators and endometrial cancer biomarkers.Cell communication and signaling : CCS · 2024Review
- Stable and reproducible MIP-ECL sensors for ultra-sensitive and accurate quantitative detection of Estrone.Frontiers in bioengineering and biotechnology · 2024Article
- Validating enzyme immunoassays for non-invasive reproductive hormone monitoring in Temminck's pangolin.Conservation physiology · 2024Article
- Critical appraisal of diagnostic laboratory tests in the evaluation of central precocious puberty.Frontiers in pediatrics · 2024Article
- Derivatization procedure of estradiol with a combination of MPDNP-F and 4-dimethylaminopyridine to generate product ion containing estradiol-skeleton for reliable determination of its serum/plasma concentrations by LC/ESI-MS/MS.Analytical and bioanalytical chemistry · 2024Article
- Development of matrix-based reference materials for 17 beta-estradiol by the recommended reference method of ID-LC-MS/MS.Analytical and bioanalytical chemistry · 2023Article
- A New Culture Model for Enhancing Estrogen Responsiveness in HR+ Breast Cancer Cells through Medium Replacement: Presumed Involvement of Autocrine Factors in Estrogen Resistance.International journal of molecular sciences · 2023Article
- Postural sway is not affected by estrogen fluctuations during the menstrual cycle.Physiological reports · 2023Article
- Rapid Detection of Estrogens in Cosmetics by Chemical Derivatization and Paper-Spray Ionization Mass-Spectrometry.Molecules (Basel, Switzerland) · 2023Article
- Role of Estradiol Hormone in Human Life and Electrochemical Aptasensing of 17β-Estradiol: A Review.Biosensors · 2022Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Estrogens and their bioactive metabolites play key roles in regulating diverse processes in health and disease. In particular, estrogens and estrogenic metabolites have shown both protective and non-protective effects on disease pathobiology, implicating the importance of this steroid pathway in disease diagnostics and monitoring. All estrogens circulate in a wide range of concentrations, which in some patient cohorts can be extremely low. However, elevated levels of estradiol are reported in disease. For example, in pulmonary arterial hypertension (PAH) elevated levels have been reported in men and postmenopausal women. Conventional immunoassay techniques have come under scrutiny, with their selectivity, accuracy and precision coming into question. Analytical methodologies such as gas and liquid chromatography coupled to single and tandem mass spectrometric approaches (GC-MS, GC-MS/MS, LC-MS and LC-MS/MS) have been developed to quantify endogenous estrogens and in some cases their bioactive metabolites in biological fluids such as urine, serum, plasma and saliva. Liquid-liquid or solid-phase extraction approaches are favoured with derivatization remaining a necessity for detection in lower volumes of sample. The limits of quantitation of individual assays vary but are commonly in the range of 0.5-5 pg/mL for estrone and estradiol, with limits for their bioactive metabolites being higher. This review provides an overview of current approaches for measurement of unconjugated estrogens in biological matrices by MS, highlighting the advances in this field and the challenges remaining for routine use in the clinical and research environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.