ArticleWestern Pacific surveillance and response journal : WPSAR
Missed detections of influenza A(H1)pdm09 by real-time RT-PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam.
Article in Western Pacific surveillance and response journal : WPSAR. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Evaluation of the STANDARD M10 Flu/RSV/SARS-CoV-2 Fast assay for the detection of influenza A/B viruses, respiratory syncytial virus, and SARS-CoV-2 in nasopharyngeal swab specimens.Microbiology spectrum · 2026Article
- Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological Advantage.Global health, epidemiology and genomics · 2022Article
- A Colorimetric Test to Differentiate Patients Infected with Influenza from COVID-19.Small structures · 2021Article
- Mutations in Animal SARS-CoV-2 Induce Mismatches with the Diagnostic PCR Assays.Pathogens (Basel, Switzerland) · 2021Article
- RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact.Frontiers in cellular and infection microbiology · 2021Article
- Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome.Royal Society open science · 2020Article
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Authors and funding
10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
introductionThere are two methods of reverse transcription polymerase chain reaction (RT-PCR) that have been the common methods to detect influenza infections: conventional and real-time RT-PCR. From December 2017 to March 2018, several missed diagnoses of influenza A(H1)pdm09 using real-time RT-PCR were reported in northern Viet Nam. This study investigated how these missed detections occurred to determine their effect on the surveillance of influenza.
methodsThe haemagglutinin (HA) segments of A(H1N1)pdm09 from both real-time RT-PCR positive and negative samples were isolated and sequenced. The primer and probe sets in the HA gene were checked for mismatches, and phylogenetic analyses were performed to determine the molecular epidemiology of these viruses.
resultsThere were 86 positive influenza A samples; 32 were A(H1)pdm09 positive by conventional RT-PCR but were negative by real-time RT-PCR. Sequencing was conducted on 23 influenza (H1N1)pdm09 isolates that were recovered from positive samples. Eight of these were negative for A(H1)pdm09 by real-time RT-PCR. There were two different mismatches in the probe target sites of the HA gene sequences of all isolates (
conclusionConstant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays.
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