ArticleNature microbiology2019
An extensively glycosylated archaeal pilus survives extreme conditions.
Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 60 citations in OpenAlex.
- Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.Engineering microbiology · 2026Review
- Cytochrome nanowires under electron microscopy.Emerging topics in life sciences · 2026Review
- Contrasting patterns for bacteria and archaea in response to salt stress across alpine wetlands of the Tibetan Plateau.Fundamental research · 2026Article
- Article
- Tat-dependent bundling pilus of a halophilic archaeon assembles by a strand donation mechanism and facilitates biofilm formation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A type IV pili-mediated mutualism between two co-resident temperate archaeal viruses and their host.Cell reports · 2025Article
- A Collagen Triple Helix without the Superhelical Twist.ACS central science · 2025Article
- Unusual cell surfaces, pili, and archaella of Thermoplasmatales archaea.The ISME journal · 2025Article
- Towards a molecular picture of the archaeal cell surface.Nature communications · 2024Article
- Structural diversity and clustering of bacterial flagellar outer domains.Nature communications · 2024Article
- A Collagen Triple Helix without the Super Helical Twist.bioRxiv : the preprint server for biology · 2024Article
- Perturbed N-glycosylation of Halobacterium salinarum archaellum filaments leads to filament bundling and compromised cell motility.Nature communications · 2024Article
- CryoEM reveals the structure of an archaeal pilus involved in twitching motility.Nature communications · 2024Article
- Two distinct archaeal type IV pili structures formed by proteins with identical sequence.Nature communications · 2024Article
- Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.The Journal of cell biology · 2023Article
- The evolution of archaeal flagellar filaments.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery.Nature communications · 2023Article
- Electron cryo-microscopy reveals the structure of the archaeal thread filament.Nature communications · 2022Article
- Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Pili on the surface of Sulfolobus islandicus are used for many functions, and serve as receptors for certain archaeal viruses. The cells grow optimally at pH 3 and ~80 °C, exposing these extracellular appendages to a very harsh environment. The pili, when removed from cells, resist digestion by trypsin or pepsin, and survive boiling in sodium dodecyl sulfate or 5 M guanidine hydrochloride. We used electron cryo-microscopy to determine the structure of these filaments at 4.1 Å resolution. An atomic model was built by combining the electron density map with bioinformatics without previous knowledge of the pilin sequence-an approach that should prove useful for assemblies where all of the components are not known. The atomic structure of the pilus was unusual, with almost one-third of the residues being either threonine or serine, and with many hydrophobic surface residues. While the map showed extra density consistent with glycosylation for only three residues, mass measurements suggested extensive glycosylation. We propose that this extensive glycosylation renders these filaments soluble and provides the remarkable structural stability. We also show that the overall fold of the archaeal pilin is remarkably similar to that of archaeal flagellin, establishing common evolutionary origins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.