ArticleNature methods2019
SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues.
Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 214 papers, 1 of them a synthesis that pooled it.
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Who cites it
214 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Enhancing RNA Capture Efficiency in Spatial Transcriptomics: A Review of Innovative Technologies and Strategies.International journal of molecular sciences · 2025Pooled it
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- Insights Into Spatial Transcriptomics: Exploring Recent Technical Developments and Their Diverse Applications.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Review
- Emerging technologies in the spatial proteomics landscape.Analytical and bioanalytical chemistry · 2026Review
- Abalone genomics reveals an ancient asymmetry axis and the multifunctionality of the mantle.Science advances · 2026Article
- PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology.Nature methods · 2026Article
- Multimodal FRACTAL-MSI and Image Fusion Improves Detection and Resolution of Biomolecule Imaging.ACS central science · 2026Article
- Spatial omics illuminates tumor heterogeneity.Cell reports methods · 2026Review
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- Programmable one-pot polymerase-mediated DNA synthesis via temperature control.Nature communications · 2026Article
- Exosome-Secreted Tropomyosin and Gigasin-6 Roles in Biomineralization Divergence Between Estuarine and Coastal Oysters.Marine biotechnology (New York, N.Y.) · 2026Article
- Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.STAR protocols · 2026Article
- Tissue rigidity phase transition shapes morphogen gradients.Nature cell biology · 2026Article
- Review
- Multiplex Fluorescent mRNA In Situ Hybridization of Developing Craniofacial Tissues in FFPE Mouse Samples.Journal of visualized experiments : JoVE · 2026Article
- A unified photosensitizer platform forbioRxiv : the preprint server for biology · 2026Article
- A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
154 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Fluorescence in situ hybridization (FISH) reveals the abundance and positioning of nucleic acid sequences in fixed samples. Despite recent advances in multiplexed amplification of FISH signals, it remains challenging to achieve high levels of simultaneous amplification and sequential detection with high sampling efficiency and simple workflows. Here we introduce signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short complementary fluorescent imager strands. We show that SABER amplified RNA and DNA FISH signals (5- to 450-fold) in fixed cells and tissues. We also applied 17 orthogonal amplifiers against chromosomal targets simultaneously and detected mRNAs with high efficiency. We then used 10-plex SABER-FISH to identify in vivo introduced enhancers with cell-type-specific activity in the mouse retina. SABER represents a simple and versatile molecular toolkit for rapid and cost-effective multiplexed imaging of nucleic acid targets.
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