ArticleNature communications2019
Tracking carrier protein motions with Raman spectroscopy.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Article
- Article
- Development of a Spectroscopic Map to Explain the Broad Raman Peak for Alkynes Solvated in Triethylamine.The journal of physical chemistry. B · 2025Article
- Determination of protein transporter function using Raman spectroscopy.Microbiology (Reading, England) · 2025Article
- Raman spectroscopy in the study of amyloid formation and phase separation.Biochemical Society transactions · 2024Review
- Frequency Changes in Terminal Alkynes Provide Strong, Sensitive, and Solvatochromic Raman Probes of Biochemical Environments.The journal of physical chemistry. B · 2023Article
- Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carrier Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Structure and mechanistic analyses of the gating mechanism of elongating ketosynthases.ACS catalysis · 2021Article
- New Immunosensing-Fluorescence Detection of Tumor Marker Cytokeratin-19 Fragment (CYFRA 21-1) Via Carbon Quantum Dots/Zinc Oxide Nanocomposite.Nanoscale research letters · 2020Article
- Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways.The Journal of biological chemistryReview
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Engineering microbial biosynthetic pathways represents a compelling route to gain access to expanded chemical diversity. Carrier proteins (CPs) play a central role in biosynthesis, but the fast motions of CPs make their conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we present a low-resource method to directly reveal carrier protein-substrate interactions. Chemoenzymatic loading of commercially available, alkyne-containing substrates onto CPs enables rapid visualization of the molecular cargo's local environment using Raman spectroscopy. This method could clarify the foundations of the chain sequestration mechanism, facilitate the rapid characterization of CPs, and enable visualization of the vectoral processing of natural products both in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.