Evidence map›Paper›PMID 31109374›Full record

ArticleStem cell research & therapy2019

Hypoxia promotes differentiation of adipose-derived stem cells into endothelial cells through demethylation of ephrinB2.

Ting Shang, Shuaijun Li, Yun Zhang, Laiya Lu, Lei Cui, Fang Fang Guo

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Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

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  12. Stem cell-mediated angiogenesis in skin tissue engineering and wound healing.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2022
    Review
  13. Article
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  17. The role of BMP4 in adipose-derived stem cell differentiation: A minireview.Frontiers in cell and developmental biology · 2022
    Review
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ting ShangDepartment of Plastic Surgery, Beijing Shijitan Hospital affiliated to Capital Medical University, 10 Tieyi Road, Beijing, China.
Shuaijun LiDepartment of Plastic Surgery, Beijing Shijitan Hospital affiliated to Capital Medical University, 10 Tieyi Road, Beijing, China.
Yun ZhangDepartment of Plastic Surgery, Beijing Shijitan Hospital affiliated to Capital Medical University, 10 Tieyi Road, Beijing, China.
Laiya LuDepartment of Orthopedics, Shanghai Tongji Hospital, Tongji University School of Medicine, 389 Xincun Road, Shanghai, China.
Lei CuiDepartment of Plastic Surgery, Beijing Shijitan Hospital affiliated to Capital Medical University, 10 Tieyi Road, Beijing, China. cuileite@aliyun.com.ORCID 0000-0002-4339-6629
Fang Fang GuoDepartment of Plastic and Reconstructive surgery, Zhongda Hospital, Southeast University, 87 Dingjiaqiao street, Nanjing, Jiangsu Province, China. anyeguofangfang@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDelivery of endothelial cells into the ischemic tissue is emerging as an alternative approach in revascularization of injured tissues by means of angiogenesis to restore organ function. Adipose-derived stem cells (ASCs) are a readily accessible source of the mesenchymal stem cell with rapid expansion and multidifferentiation potential. The view has emerged that endothelial cells (ECs) differentiated from ASCs is a step forward for adult vascular repair in regenerative medicine and construction of the blood vessel by tissue engineering approach.

methodsIn this study, differentiation of human ASCs (hASCs) into vascular EC lineage was induced by combined treatment of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-4 (BMP4) under hypoxia condition. The expression of CD31, VEGF-R2, and VE-cadherin was determined by immunofluorescent staining, real-time PCR, and western blot analysis. These differentiated cells acquired functional characteristics of mature ECs as determined by their tube formation ability, DiI-ac-LDL uptake, and nitric oxide secretion in vitro. The methylation status in the proximal promoter CpGs was determined by bisulfite sequencing.

resultshASCs expressed endothelial cell markers including CD31, VEGF-R2, and VE-cadherin by combined treatment of VEGF and BMP4 under hypoxia condition. These differentiated cells exhibited the angiogenesis potential in vitro, and injection of these differentiated cells enhanced angiogenesis in the ischemic hindlimb of diabetic mice. Furthermore, it was found that hypoxia increased significantly EphrinB2 expression EC differentiation, which is greatly downregulated with EphrinB2 blockage. The methylation status in the proximal promoter CpG results showed that methylation of EphrinB2 promoter decreased in hASCs with exposure to hypoxia.

conclusionOur data demonstrate that hASCs can be efficiently induced to differentiate into vascular EC lineages which are mediated by demethylation of ephrinB2 under hypoxia condition.

Indexed as

Adipose TissueAnimalsCell DifferentiationCell HypoxiaCells, CulturedEndothelial CellsEphrin-B2FemaleHumansMesenchymal Stem CellsMiceEphrin-B2Adipose-derived stem cellsDemethylationephrinB2HypoxiaVascular endothelial cell

Identifiers

PMID31109374
PMCPMC6528245

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