ArticleStem cell research & therapy2019
Xenotransplantation of canine spermatogonial stem cells (cSSCs) regulated by FSH promotes spermatogenesis in infertile mice.
Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 15 citations in OpenAlex.
- The gut-brain-gonad axis mediates salinity adaptation in an invasive turtle: causal evidence from microbiota transplantation and metabolite supplementation.Animal microbiome · 2026Article
- Spermatogonial Stem Cells in Domestic Animals: Current Insights and Future Directions with a Focus on Dogs.Veterinary sciences · 2025Review
- Testicular cryopreservation: From technical aspects to practical applications.Histology and histopathology · 2025Review
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation†.Biology of reproduction · 2024Review
- Co-Culture of Cryopreserved Healthy Sertoli Cells with Testicular Tissue of Non-Obstructive Azoospermia (NOA) Patients in Culture Media Containing Follicle-Stimulating Hormone (FSH)/Testosterone Has No Advantage in Germ Cell Maturation.Journal of clinical medicine · 2023Article
- Detection of spermatogonial stem cells in testicular tissue of dogs with chronic asymptomatic orchitis.Frontiers in veterinary science · 2023Article
- A novel role of follicle-stimulating hormone (FSH) in various regeneration-related functions of endometrial stem cells.Experimental & molecular medicine · 2022Article
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Actions and Roles of FSH in Germinative Cells.International journal of molecular sciences · 2021Review
- Direct action of FSH on testicular stem cells.Stem cell research & therapy · 2019Article
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11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundXenotransplantation of spermatogonial stem cells (SSCs) has become a popular topic in various research fields because manipulating these cells can provide insights into the mechanisms associated with germ cell lines and the entire spermatogenesis process; moreover, these cells can be used in several biotechnology applications. To achieve successful xenotransplantation, the in vitro microenvironment in which SSCs are cultured should be an ideal microenvironment for self-renewal and similar to the in vivo testicular microenvironment. The age of the donor, the correct spermatogenesis cycle, and the quality of the donor tissue are also important. Although cell culture-related factors, such as the in vitro supplementation of hormonal factors, are known to promote successful xenotransplantation in mice, little is known about the influence of these factors on SSCs in vitro or in vivo in other mammalian species, such as dogs (Canis lupus familiaris). In this context, the goals of this study were to test the effect of follicle-stimulating hormone (FSH) on canine spermatogonial stem cell (cSSC) cultures since this hormone is related to the glial cell-derived neurotrophic factor (GDNF) signaling pathway, which is responsible for the self-renewal and maintenance of these cells in vivo, and to investigate the microenvironment of the SSC culture after FSH supplementation. Additionally, in vivo analyses of transplanted FSH-supplemented cSSCs in the testes of infertile mice were performed to assess the capacity of cSSCs to develop, maintain, and restore spermatogenesis.
methodsSSCs from canine prepubertal testes (aged 3 months) were cultured in vitro in the presence of FSH (10 IU L
resultsSupplementation with FSH in cell culture increased the number of cSSCs positive for GFRA1. The cSSCs were also positive for the pluripotency and early germline marker OCT4 and the late germline markers PLZF, DAZL, C-kit, and GFRA-1. The in vivo experiments showed that the cSSCs xenotransplanted into infertile mouse testes were able to repopulate germline cells in the seminiferous tubules of mice.
conclusionsIn conclusion, our results showed for the first time that the treatment of cSSC cultures with FSH can promote in vitro self-renewal, increase the population of germline cells, and possibly influence the success of spermatogenesis in infertile mice in vivo.
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