ArticleMetabolites2019
Effects of Long-Term Storage at -80 °C on the Human Plasma Metabolome.
Article in Metabolites, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
72 citing papers in PubMed.
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- Predictive utilities of lipid traits, lipoprotein subfractions and other risk factors for incident diabetes: a machine learning approach in the Diabetes Prevention Program.BMJ open diabetes research & care · 2021Trial
- Machine learning reveals serum sphingolipids as cholesterol-independent biomarkers of coronary artery disease.The Journal of clinical investigation · 2020Trial
- Metabolic Effects of Healing Touch During Cervical Cancer Treatment: An Exploratory Analysis.Integrative cancer therapiesTrial
- Article
- Mass Spectrometry-Based Chromatographic and Computational Workflows for Biomarker and Therapeutic Target Discovery: A Comprehensive Review.Biomedical chromatography : BMC · 2026Review
- Stage-Associated Physicochemical and Chemical Profiles of a Single Production Run ofFoods (Basel, Switzerland) · 2026Article
- Determinants of the plasma metabolome: cross-sectional and longitudinal associations over six years in the NESDA cohort.EBioMedicine · 2026Article
- Systematic Evaluation of Plasma and Urine Metabolites to Predict the Risk of Adverse Kidney-related Outcomes in Chronic Kidney Disease: The GCKD Study∗.Kidney medicine · 2026Article
- Effects of Oral Care Intervention on Gingivitis-Mediated Induction of Nitric Oxide Synthesis and Oxidative Stress.International journal of molecular sciences · 2026Article
- Plasma short-chain fatty acid concentrations in social anxiety disorder and changes after cognitive behavioral therapy.Translational psychiatry · 2026Article
- Metabolic and lipidomic stability in EDTA plasma from hospitalized patients: impact of refrigerated storage time and additional freeze-thaw cycle.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Untargeted Metabolomics Analysis of Human Milk from Breastfeeding Bangladeshi Women Reveals Amino Acid Metabolic Pathways Associated with Maternal Nutritional Status and Infant Growth.The Journal of nutrition · 2026Article
- Adhesion molecules in the maternal-fetal interface in healthy pregnancies and preeclampsia.Acta obstetricia et gynecologica Scandinavica · 2026Article
- Low Serum Lysophospholipids Predict Increased In-Hospital Mortality in Patients With Acute Heart Failure.Journal of the American Heart Association · 2026Observational
- A comparison of sequential density ultracentrifugation and density gradient ultracentrifugation for the proteomic analysis of high-density lipoprotein.Frontiers in cardiovascular medicine · 2026Article
- Monitoring of Allograft Adaptation After Kidney Transplantation in Pediatric Patients by Targeted Plasma Metabolomics.International journal of molecular sciences · 2025Article
- TRAIL and RIPK3 Do Not Predict Mortality and Acute Kidney Injury in Critically Ill Patients at Admission to the Intensive Care Unit-A Single-Center Cohort Study.International journal of molecular sciences · 2025Article
- An untargeted metabolomics analysis suggests pathophysiological changes for post cardiac arrest syndrome.Resuscitation plus · 2025Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-quality biological samples are required for the favorable outcome of research studies, and valid data sets are crucial for successful biomarker identification. Prolonged storage of biospecimens may have an artificial effect on compound levels. In order to investigate the potential effects of long-term storage on the metabolome, human ethylenediaminetetraacetic acid (EDTA) plasma samples stored for up to 16 years were analyzed by gas and liquid chromatography-tandem mass spectrometry-based metabolomics. Only 2% of 231 tested plasma metabolites were altered in the first seven years of storage. However, upon longer storage periods of up to 16 years and more time differences of few years significantly affected up to 26% of the investigated metabolites when analyzed within subject age groups. Ontology classes that were most affected included complex lipids, fatty acids, energy metabolism molecules, and amino acids. In conclusion, the human plasma metabolome is adequately stable to long-term storage at -80 °C for up to seven years but significant changes occur upon longer storage. However, other biospecimens may display different sensitivities to long-term storage. Therefore, in retrospective studies on EDTA plasma samples, analysis is best performed within the first seven years of storage.
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