ArticleMicroscopy research and technique2019
Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips).
Article in Microscopy research and technique, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Traceable stiffness calibration of colloidal AFM probes for biomechanical measurements.Scientific reports · 2026Article
- Comparison of viscoelastic properties of breast cancer and normal cells using AFM, FLIM and ToF-SIMS techniques.Scientific reports · 2025Article
- Mechanical Properties of Endothelial Cells: A Key to Physiology, Drug Testing and Nanostructure Interaction.Cells · 2025Review
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- Elasticity, an often-overseen parameter in the development of nanoscale drug delivery systems.Beilstein journal of nanotechnology · 2023Article
- Nucleotide-Induced Nanoscale Changes in the Mechanical Properties of Rat Cerebellar Astrocytes: Selective Stimulation and Blocking of the Purinergic Receptor P2X7.International journal of molecular sciences · 2022Article
- Measuring biological materials mechanics with atomic force microscopy - Determination of viscoelastic cell properties from stress relaxation experiments.Microscopy research and technique · 2022Article
- A-to-I RNA editing of Filamin A regulates cellular adhesion, migration and mechanical properties.The FEBS journal · 2022Article
- The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro.Journal of microscopy · 2022Article
- Reduced platelet forces underlie impaired hemostasis in mouse models ofScience advances · 2022Article
- Intranuclear HSV-1 DNA ejection induces major mechanical transformations suggesting mechanoprotection of nucleus integrity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Action of Mechanical Forces on Polymerization and Polymers.Polymers · 2022Review
- Reconstituted virus-nucleus system reveals mechanics of herpesvirus genome uncoating.QRB discovery · 2022Article
- Estrogen Modulates Epithelial Breast Cancer Cell Mechanics and Cell-to-Cell Contacts.Materials (Basel, Switzerland) · 2021Article
- Nucleotides-Induced Changes in the Mechanical Properties of Living Endothelial Cells and Astrocytes, Analyzed by Atomic Force Microscopy.International journal of molecular sciences · 2021Article
- Article
- Indenting soft samples (hydrogels and cells) with cantilevers possessing various shapes of probing tip.European biophysics journal : EBJ · 2020Article
- Microtubule disruption changes endothelial cell mechanics and adhesion.Scientific reports · 2019Article
- Measuring biomaterials mechanics with atomic force microscopy. 1. Influence of the loading rate and applied force (pyramidal tips).Microscopy research and technique · 2019Article
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4 authors.
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Abstract
Atomic force microscopy (AFM) is today an established tool in imaging and determination of mechanical properties of biomaterials. Due to their complex organization, those materials show intricate properties such as viscoelasticity. Therefore, one has to consider that the loading rate at which the sample is probed will lead to different mechanical response (properties). In this work, we studied the dependence of the mechanical properties of endothelial cells on the loading rate using AFM in force spectroscopy mode. We employed a sharp, four-sided pyramidal indenter and loading rates ranging from 0.5 to 20 μm/s. In addition, by variation of the load (applied forces from 100 to 10,000 pN), the dependence of the cell properties on indentation depth could be determined. We then showed that the mechanical response of endothelial cells depends nonlinearly on the loading rate and follows a weak power-law. In addition, regions of different viscous response at varying indentation depth could be determined. Based on the results we obtained, a general route map for AFM users for design of cell mechanics experiments was described.
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