ArticleNucleic acids research2019
Rapid functional activation of a horizontally transferred eukaryotic gene in a bacterial genome in the absence of selection.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- A hidden intrinsic ability of bicistronic expression based on a novel translation reinitiation mechanism in yeast.Nucleic acids research · 2025Article
- Giant viruses as reservoirs of antibiotic resistance genes.Nature communications · 2024Article
- Rapid functional activation of horizontally transferred eukaryotic intron-containing genes in the bacterial recipient.Nucleic acids research · 2024Article
- Tandem gene duplication selected by activation of horizontally transferred gene in bacteria.Applied microbiology and biotechnology · 2024Article
- Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant molecular biology · 2024Article
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Natural bacterial isolates as an inexhaustible source of new bacteriocins.Applied microbiology and biotechnology · 2021Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Horizontal gene transfer has occurred between organisms of all domains of life and contributed substantially to genome evolution in both prokaryotes and eukaryotes. Phylogenetic evidence suggests that eukaryotic genes horizontally transferred to bacteria provided useful new gene functions that improved metabolic plasticity and facilitated adaptation to new environments. How these eukaryotic genes evolved into functional bacterial genes is not known. Here, we have conducted a genetic screen to identify the mechanisms involved in functional activation of a eukaryotic gene after its transfer into a bacterial genome. We integrated a eukaryotic selectable marker gene cassette driven by expression elements from the red alga Porphyridium purpureum into the genome of Escherichia coli. Following growth under non-selective conditions, gene activation events were indentified by antibiotic selection. We show that gene activation in the bacterial recipient occurs at high frequency and involves two major types of spontaneous mutations: deletion and gene amplification. We further show that both mechanisms result in promoter capture and are frequently triggered by microhomology-mediated recombination. Our data suggest that horizontally transferred genes have a high probability of acquiring functionality, resulting in their maintenance if they confer a selective advantage.
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