Evidence map›Paper›PMID 31106236›Full record

ArticleBio-protocol2019

Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices.

Victor S Van Laar, Beth Arnold, Sarah B Berman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Victor S Van LaarUniversity of Pittsburgh Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Beth ArnoldUniversity of Pittsburgh Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Sarah B BermanUniversity of Pittsburgh Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213, USA.

Funding

Neuronal regulation of mitochondrial dynamics in models of Parkinson's disease.R01NS077954 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BERMAN, SARAH B · 2012 to 2016
$1.7M
NINDS NIH HHS R01 NS077954
6 · The paper itself

Abstract

In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic needs of primary neurons present complications for their study in culture. Recent microculture techniques utilizing microfluidic culture devices allows for environmental separation and analysis of neuronal cell bodies and neurites in culture. Here, we present our protocol for culture of primary neurons in microfluidic devices and their chronic treatment with the Parkinson's disease (PD) relevant toxicant rotenone. In addition, we present a method for reuse of devices for culture. This culture methodology presents advantages for evaluating early pathogenic cellular and molecular changes in neurons in a compartment-specific manner.

Indexed as

Microfluidic chamberMicrofluidic cultureMicrofluidic deviceMicrofluidic device reuseParkinson’s diseasePrimary neuron cultureRotenone

Identifiers

PMID31106236
PMCPMC6519463

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.