ArticleInsect biochemistry and molecular biology2019
Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.
Article in Insect biochemistry and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Evaluation of mosquito transmission of equine hepacivirus in laboratory and field studies.Journal of medical entomology · 2026Article
- Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response.Nature communications · 2026Article
- Furin as target for suppression of mosquito-borne viruses.Virology journal · 2026Article
- Laboratory evidence on the vector competence of European field-captured Culex theileri for circulating West Nile virus lineages 1 and 2.Parasites & vectors · 2025Article
- The Hsf1-sHsp cascade has pan-antiviral activity in mosquito cells.Communications biology · 2025Article
- Article
- Immune-mediated competition benefits protective microbes over pathogens in a novel host species.Heredity · 2022Article
- Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes.Journal of visualized experiments : JoVE · 2022Article
- Juvenile Hormone-Sensitive Ribosomal Activity Enhances Viral Replication in Aedes aegypti.mSystems · 2021Article
- The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in TransgenicViruses · 2020Article
- Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission.Journal of virology · 2020Article
- Advances in oral RNAi for disease vector mosquito research and control.Current opinion in insect science · 2020Review
- Review
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
The yellow fever mosquito, Aedes aegypti, serves as the primary vector for epidemic transmission of yellow fever, dengue, Zika (ZIKV), and chikungunya viruses to humans. Control of Ae. aegypti is currently limited to insecticide applications and larval habitat management; however, to combat growing challenges with insecticide resistance, novel genetic approaches for vector population reduction or transmission interruption are being aggressively pursued. The objectives of this study were to assess the ability of the Ae. aegypti antiviral exogenous-small interfering RNA (exo-siRNA) response to inhibit ZIKV infection and transmission, and to identify the optimal RNA interference (RNAi) target region in the ZIKV genome. We accomplished these objectives by in vitro transcription of five long double-stranded RNAs (dsRNAs) from the genome region spanning the NS2B-NS3-NS4A genes, which were the most highly conserved among ZIKV RNA sequences representing both East and West African and Asian-American clades, and evaluation of the ability of these dsRNAs to trigger an effective antiviral exo-siRNA response after intrathoracic injection into Ae. aegypti. In a pilot study, five ZIKV dsRNAs were tested by intrathoracic inoculation of 250 ng dsRNA into groups of approximately 5-day-old mosquitoes. Three days post-inoculation, mosquitoes were provided an infectious blood-meal containing ZIKV strain PRVABC59 (Puerto Rico), MR766 (Uganda), or 41525 (Senegal). On days 7 and 14 post-infection individual whole mosquito bodies were assessed for ZIKV infectious titer by plaque assays. Based on the results of this initial assessment, three dsRNAs were selected for further evaluation of viral loads of matched body and saliva expectorants using a standardized infectious dose of 1 × 10
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