ArticleMicrobial cell factories2019
Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Effects of Replacing Alfalfa withAnimals : an open access journal from MDPI · 2026Article
- Engineering of xylose metabolic pathways in Rhodotorula toruloides for sustainable biomanufacturing.FEMS yeast research · 2025Review
- Review
- A Novel Biosynthetic Strategy for Ginsenoside Ro: Construction of a Metabolically EngineeredInternational journal of molecular sciences · 2024Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Advances in the biosynthesis and metabolic engineering of rare ginsenosides.Applied microbiology and biotechnology · 2023Review
- Microorganisms for Ginsenosides Biosynthesis: Recent Progress, Challenges, and Perspectives.Molecules (Basel, Switzerland) · 2023Review
- Engineering ofMetabolic engineering communications · 2022Review
- Lipid production from lignocellulosic biomass using an engineered Yarrowia lipolytica strain.Microbial cell factories · 2022Article
- Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose.Biotechnology for biofuels and bioproducts · 2022Article
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineeredFrontiers in microbiology · 2022Article
- Research progress in bioremediation of petroleum pollution.Environmental science and pollution research international · 2021Review
- Recent Advances in Producing Sugar Alcohols and Functional Sugars by EngineeringFrontiers in bioengineering and biotechnology · 2021Review
- Yeast-Based Biosynthesis of Natural Products From Xylose.Frontiers in bioengineering and biotechnology · 2021Review
- Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform.Journal of industrial microbiology & biotechnology · 2020Review
- Review
- Application of Stable Isotope Tracing to Elucidate Metabolic Dynamics During Yarrowia lipolytica α-Ionone Fermentation.iScience · 2020Article
- Metabolic engineering ofBiotechnology for biofuels · 2020Article
- Article
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6 authors.
Funding
Abstract
backgroundAs renewable biomass, lignocellulose remains one of the major choices for most countries in tackling global energy shortage and environment pollution. Efficient utilization of xylose, an important monosaccharide in lignocellulose, is essential for the production of high-value compounds, such as ethanol, lipids, and isoprenoids. Protopanaxadiol (PPD), a kind of isoprenoids, has important medical values and great market potential.
resultsThe engineered protopanaxadiol-producing Yarrowia lipolytica strain, which can use xylose as the sole carbon source, was constructed by introducing xylose reductase (XR) and xylitol dehydrogenase (XDH) from Scheffersomyces stipitis, overexpressing endogenous xylulose kinase (ylXKS) and heterologous PPD synthetic modules, and then 18.18 mg/L of PPD was obtained. Metabolic engineering strategies such as regulating cofactor balance, enhancing precursor flux, and improving xylose metabolism rate via XR (K270R/N272D) mutation, the overexpression of tHMG1/ERG9/ERG20 and transaldolase (TAL)/transketolase (TKL)/xylose transporter (TX), were implemented to enhance PPD production. The final Y14 strain exhibited the greatest PPD titer from xylose by fed-batch fermentation in a 5-L fermenter, reaching 300.63 mg/L [yield, 2.505 mg/g (sugar); productivity, 2.505 mg/L/h], which was significantly higher than the titer of glucose fermentation [titer, 167.17 mg/L; yield, 1.194 mg/g (sugar); productivity, 1.548 mg/L/h].
conclusionThe results showed that xylose was more suitable for PPD synthesis than glucose due to the enhanced carbon flux towards acetyl-CoA, the precursor for PPD biosynthetic pathway. This is the first report to produce PPD in Y. lipolytica with xylose as the sole carbon source, which developed a promising strategy for the efficient production of high-value triterpenoid compounds.
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