ArticleScientific reports2019
Effect of mycalolides isolated from a marine sponge Mycale aff. nullarosette on actin in living cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
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- Discovery of a lagriamide polyketide by integrated genome mining, isotopic labeling, and untargeted metabolomics.Chemical science · 2024Article
- Tunneling Nanotubes in the Brain.Results and problems in cell differentiation · 2024Review
- Review
- Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (Membranes · 2021Article
- Bioactive Compounds from Marine Sponges: Fundamentals and Applications.Marine drugs · 2021Review
- Histone modification dynamics as revealed by multicolor immunofluorescence-based single-cell analysis.Journal of cell science · 2020Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
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Abstract
Discovery of novel bioactive compounds is important not only for therapeutic purposes but also for understanding the mechanisms of biological processes. To screen bioactive compounds that affect nuclear morphology in marine organism extracts, we employed a microscopy-based assay using DNA staining of human cancer cells. A crude extract from a marine sponge Mycale aff. nullarosette, collected from the east coast of Japan, induced cellular binucleation. Fractionation of the extract led to the isolation of mycalolides A and B, and 38-hydroxymycalolide B as the active components. Mycalolides have been identified as marine toxins that induce depolymerization of the actin filament. Live cell imaging revealed that low concentrations of mycalolide A produce binucleated cells by inhibiting the completion of cytokinesis. At higher concentrations, however, mycalolide A causes immediate disruption of actin filaments and changes in cell morphology, yielding rounded cells. These results suggest that the completion of cytokinesis is a process requiring high actin polymerization activity. Furthermore, luciferase reporter assays with mycalolide A treatments support the view that the level of globular actin can affect transcription of a serum response gene.
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