ArticleScientific reports2019
GFP fluorescence peak fraction analysis based nanothermometer for the assessment of exothermal mitochondria activity in live cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Angle-based fluorescence thermometry with high sensitivity and high resolution.Nature communications · 2026Article
- Hot Mitochondria and the Second Law of Thermodynamics.Research square · 2026Article
- Single-Nanoparticle Luminescence Nanothermometers with Enhanced Sensitivity in Physiological Temperature Range.ACS omega · 2025Article
- β-Cyclodextrin-Grafted Polypyrrole-Rhodamine B Nanoplatforms for Drug Delivery and Image-Guided Photothermal Therapy In Vitro.Materials (Basel, Switzerland) · 2025Article
- Chemically Anchored Diamond with H3 Centers for Ratiometric Measurement of Isolated Mitochondria Temperature.International journal of molecular sciences · 2025Article
- Exploring Water Beyond the Solvent: Insights into Density Fluctuations and EGFP Unfolding via Luminescence Thermometry.The journal of physical chemistry. B · 2025Article
- Comparative Approaches for Quantification of Product Yield in the Production of Recombinant Green Fluorescent Protein (GFPuv) Expressed inbioRxiv : the preprint server for biology · 2025Article
- Simultaneous Detection of Neural Activity and Temperature in Photothermal Neural Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Mitochondria: great balls of fire.The FEBS journal · 2024Review
- Heat application in live cell imaging.FEBS open bio · 2024Review
- Effective and stable gene transduction in rhesus macaque iPSCs capable of T-lineage differentiation utilizing the piggyBac system.Regenerative therapy · 2024Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Multimodal Temperature Readout Boosts the Performance of CuInSACS applied materials & interfaces · 2024Article
- TRPV2: a universal regulator in cellular physiology with a yet poorly defined thermosensitivity.The journal of physiological sciences : JPS · 2024Review
- Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level.ACS sensors · 2024Article
- Thermal properties of nanofluids using hydrophilic and hydrophobic LiYFNanoscale advances · 2024Article
- Article
- Warm Cells, Hot Mitochondria: Achievements and Problems of Ultralocal Thermometry.International journal of molecular sciences · 2023Review
- Luminescence Thermometry with Nanoparticles: A Review.Nanomaterials (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanothermometry methods with intracellular sensitivities have the potential to make important contributions to fundamental cell biology and medical fields, as temperature is a relevant physical parameter for molecular reactions to occur inside the cells and changes of local temperature are well identified therapeutic strategies. Here we show how the GFP can be used to assess temperature-based on a novel fluorescence peak fraction method. Further, we use standard GFP transfection reagents to assess temperature intracellularly in HeLa cells expressing GFP in the mitochondria. High thermal resolution and sensitivity of around 0.26% °C
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.