ArticleProceedings of the National Academy of Sciences of the United States of America2019
A human organoid system that self-organizes to recapitulate growth and differentiation of a benign mammary tumor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Construction and application of mammary and breast cancer organoids.Cell insight · 2026Review
- Markers of immune activation and immunotherapy responsiveness are increased in 3D Pancreatic cancer organoids when primed with photodynamic- and chemo-therapy.Translational oncology · 2026Article
- Gap junction communication regulates luminal-myoepithelial crosstalk and cell differentiation in a bilayered human mammary epithelial cell model.Biology methods & protocols · 2026Article
- Dynamic culture system advances the applications of breast cancer organoids for precision medicine.Scientific reports · 2025Article
- Tumoroids, a valid preclinical screening platform for monitoring cancer angiogenesis.Stem cell research & therapy · 2024Review
- The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma.Cell death & disease · 2024Article
- Light and electron microscopy continuum-resolution imaging of 3D cell cultures.Developmental cell · 2023Article
- State-dependent evolutionary models reveal modes of solid tumour growth.Nature ecology & evolution · 2023Article
- Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination.Frontiers in oncology · 2022Review
- A novel paper MAP method for rapid high resolution histological analysis.Scientific reports · 2021Article
- The molecular basis of mammary gland development and epithelial differentiation.Seminars in cell & developmental biology · 2021Review
- Cancer Cell Invasion of Mammary Organoids with Basal-In Phenotype.Advanced healthcare materials · 2021Article
- Recent advances in understanding the role of metabolic heterogeneities in cell migration.Faculty reviews · 2021Review
- Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages.Nature communications · 2020Article
- Crowds and power - coordinated in vitro development of a benign breast lesion.Molecular & cellular oncology · 2019Article
Corrections and comments
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Authors and funding
5 authors.
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Abstract
As 3D culture has become central to investigation of tissue biology, mammary epithelial organoids have emerged as powerful tools for investigation of epithelial cell polarization and carcinogenesis. However, most current protocols start from single cells suspended in Matrigel, which can also restrict cell differentiation and behavior. Here, we show that the noncancerous mammary cell line HMT-3522 S1, when allowed to spontaneously form cell aggregates ("spheroids") in medium without Matrigel, switches to a collective growth mode that recapitulates many attributes of "usual ductal hyperplasia" (UDH), a common benign mammary lesion. Interestingly, these spheroids undergo a complex maturation process reminiscent of embryonic development: solid-cell cords form their own basement membrane, grow on the surface of initially homogeneous cell aggregates, and form asymmetric lumina lined by two distinct cell types that express basal and luminal cytokeratins. This sequence of events provides a cellular mechanism that explains how the characteristic crescent-shaped, asymmetrical lumina form in UDH. Our results suggest that HMT-3522 S1 spheroids are useful as an in vitro model system to study UDH biology, glandular lumen formation, and stem cell biology of the mammary gland.
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