ArticleProceedings of the National Academy of Sciences of the United States of America2019
Microsecond and millisecond dynamics in the photosynthetic protein LHCSR1 observed by single-molecule correlation spectroscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 46 citations in OpenAlex.
- LHCSR1 Functions as a Dimmer Switch for Light Harvesting.The journal of physical chemistry letters · 2026Article
- Two-dimensional bond-selective fluorescence spectroscopy: violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging.Chemical science · 2025Article
- Evidence of a Three-State Mechanism in DNA Hairpin Folding.Journal of the American Chemical Society · 2025Article
- Development of Molecular Dynamics Parameters and Theoretical Analysis of Excitonic and Optical Properties in the Light-Harvesting Complex II.Journal of chemical theory and computation · 2025Article
- pH-Dependent Assembly and Stability of Toll-Like Receptor 3/dsRNA Signaling Complex: Insights from Constant pH Molecular Dynamics and Metadynamics Simulations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Quantifying Microsecond Solution-Phase Conformational Dynamics of a DNA Hairpin at the Single-Molecule Level.ACS physical chemistry Au · 2024Article
- Ultrafast energy quenching mechanism of LHCSR3-dependent photoprotection in Chlamydomonas.Nature communications · 2024Article
- Pulsed-Interleaved-Excitation Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy.The journal of physical chemistry. B · 2024Article
- The nature of carotenoid S* state and its role in the nonphotochemical quenching of plants.Nature communications · 2024Article
- How the pH Controls Photoprotection in the Light-Harvesting Complex of Mosses.Journal of the American Chemical Society · 2023Article
- Structure of the stress-related LHCSR1 complex determined by an integrated computational strategy.Communications biology · 2022Article
- A different perspective for nonphotochemical quenching in plant antenna complexes.Nature communications · 2021Article
- Fast and robust two-dimensional inverse Laplace transformation of single-molecule fluorescence lifetime data.Biophysical journal · 2021Article
- Membrane-dependent heterogeneity of LHCII characterized using single-molecule spectroscopy.Biophysical journal · 2021Article
- Article
- Lipid and protein dynamics of stacked and cation-depletion induced unstacked thylakoid membranes.BBA advances · 2021Article
- Charge transfer from the carotenoid can quench chlorophyll excitation in antenna complexes of plants.Nature communications · 2020Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- Single-molecule FRET methods to study the dynamics of proteins at work.Current opinion in biomedical engineering · 2019Article
- Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana.Photosynthesis research · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
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Abstract
Biological systems are subjected to continuous environmental fluctuations, and therefore, flexibility in the structure and function of their protein building blocks is essential for survival. Protein dynamics are often local conformational changes, which allows multiple dynamical processes to occur simultaneously and rapidly in individual proteins. Experiments often average over these dynamics and their multiplicity, preventing identification of the molecular origin and impact on biological function. Green plants survive under high light by quenching excess energy, and Light-Harvesting Complex Stress Related 1 (LHCSR1) is the protein responsible for quenching in moss. Here, we expand an analysis of the correlation function of the fluorescence lifetime by improving the estimation of the lifetime states and by developing a multicomponent model correlation function, and we apply this analysis at the single-molecule level. Through these advances, we resolve previously hidden rapid dynamics, including multiple parallel processes. By applying this technique to LHCSR1, we identify and quantitate parallel dynamics on hundreds of microseconds and tens of milliseconds timescales, likely at two quenching sites within the protein. These sites are individually controlled in response to fluctuations in sunlight, which provides robust regulation of the light-harvesting machinery. Considering our results in combination with previous structural, spectroscopic, and computational data, we propose specific pigments that serve as the quenching sites. These findings, therefore, provide a mechanistic basis for quenching, illustrating the ability of this method to uncover protein function.
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