Evidence map›Paper›PMID 31100976›Full record

ArticleMolecules (Basel, Switzerland)2019

Oriented Immobilization and Quantitative Analysis Simultaneously Realized in Sandwich Immunoassay via His-Tagged Nanobody.

Li Xu, Hanyu Cao, Chundong Huang, Lingyun Jia

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Direct Immobilization of Engineered Nanobodies on Gold Sensors.ACS applied materials & interfaces · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Li XuLiaoning Key Laboratory of Molecular Recognition and imaging, School of Bioengineering, Dalian University of Technology, No.2 Linggong Road, Dalian, Liaoning 116023, China. xu-li@dlut.edu.cn.
Hanyu Cao
Chundong Huang
Lingyun Jia
Dalian University · CNDalian University of Technology · CN

Funding

National Key Technologies R and D Peogress,China 2016YFC1103000National Natural Science Foundation of China 81671073
6 · The paper itself

Abstract

Despite the advantages of the nanobody, the unique structure limits its use in sandwich immunoassay. In this study, a facile protocol of sandwich immunoassay using the nanobody was established. In brief, β amyloid and SH2, an anti-β amyloid nanobody, were used as capture antibody and antigen, respectively. The SH2 fused with His-tag was first purified and absorbed on Co

Indexed as

ImmunoassayRecombinant Fusion ProteinsGene ExpressionHydrogen PeroxideOxidation-ReductionSingle-Domain Antibodiessrc Homology DomainsHydrogen PeroxideRecombinant Fusion ProteinsSingle-Domain AntibodiesHis-tagnanobodyoriented immobilizationsandwich immunoassayβ amyloid detection

Identifiers

PMID31100976
PMCPMC6572564
OpenAlexW2945210488

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.