ArticleInternational journal of molecular sciences2019
Extra Purified Exosomes from Human Placenta Contain An Unpredictable Small Number of Different Major Proteins.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Harnessing Mammalian- and Plant-Derived Exosomes for Drug Delivery: A Comparative Review.International journal of molecular sciences · 2025Review
- Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution.Nature communications · 2025Article
- A simple capillary isoelectric focusing method as the novel strategy for the isoelectric point measurement of exosomes and its application in disease diagnosis.Mikrochimica acta · 2024Article
- Challenges of MS-based small extracellular vesicles proteomics.Journal of extracellular vesicles · 2024Review
- Bile from the hemojuvelin-deficient mouse model of iron excess is enriched in iron and ferritin.Metallomics : integrated biometal science · 2024Article
- Analysis and Characterization of the Extracellular Vesicles Released in Non-Cancer Diseases Using Matrix-Assisted Laser Desorption Ionization/Mass Spectrometry.International journal of molecular sciences · 2024Review
- Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification.Beilstein journal of nanotechnology · 2024Article
- Isolation of Extracellular Vesicles of Holothuria (Sea CucumberInternational journal of molecular sciences · 2023Article
- Circulating microRNAs as emerging regulators of COVID-19.Theranostics · 2023Review
- Analysis of Proteins and Peptides of Highly Purified CD9International journal of molecular sciences · 2022Article
- Applications of MALDI-MS/MS-Based Proteomics in Biomedical Research.Molecules (Basel, Switzerland) · 2022Review
- Peptide ligand-SiOBioactive materials · 2022Article
- The role of extracellular vesicles in animal reproduction and diseases.Journal of animal science and biotechnology · 2022Review
- Potential Applications and Functional Roles of Exosomes in Cardiometabolic Disease.Pharmaceutics · 2021Review
- miR-335-laden B Cell-Derived Extracellular Vesicles Promote SOX4-Dependent Apoptosis in Human Multiple Myeloma Cells.Journal of personalized medicine · 2021Article
- [Proteomic analysis of serum and serum exosomes, and their application in intrahepatic cholangiocarcinoma].Se pu = Chinese journal of chromatography · 2021Article
- Review of Methodological Approaches to Human Milk Small Extracellular Vesicle Proteomics.Biomolecules · 2021Review
- Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs.Journal of extracellular vesicles · 2021Article
- Pregnancy-Related Extracellular Vesicles Revisited.International journal of molecular sciences · 2021Review
- Immunosuppressive Effects of Mesenchymal Stem Cells-derived Exosomes.Stem cell reviews and reports · 2021Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exosomes are nanovesicles (30-100 nm) containing various RNAs and different proteins. Exosomes are important in intracellular communication, immune function, etc. Exosomes from different sources including placenta were mainly obtained by different types of centrifugation and ultracentrifugations and were reported to contain from a few dozen to thousands of different proteins. First crude exosome preparations from four placentas (normal pregnancy) were obtained here using several standard centrifugations but then were additionally purified by gel filtration on Sepharose 4B. Individual preparations demonstrated different gel filtration profiles showing good or bad separation of exosome peaks from two peaks of impurity proteins and their complexes. According to electron microscopy, exosomes before gel filtration contain vesicles of different size, ring-shaped structures forming by ferritin and clusters of aggregated proteins and their complexes. After filtration through 220 nm filters and gel filtration exosomes display typically for exosome morphology and size (30-100 nm) and do not contain visible protein admixtures. Identification of exosome proteins was carried out by MS and MS/MS MALDI mass spectrometry of proteins' tryptic hydrolyzates after their SDS-PAGE and 2D electrophoresis. We have obtained unexpected results. Good, purified exosomes contained only 11-13 different proteins: CD9, CD81, CD-63, hemoglobin subunits, interleukin-1 receptor, annexin A1, annexin A2, annexin A5, cytoplasmic actin, alkaline phosphatase, serotransferin, and probably human serum albumin and immunoglobulins. We assume that a possible number of exosome proteins found previously using crude preparations may be very much overestimated. Our data may be important for study of biological functions of pure exosomes.
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