ArticlePLoS biology2019
Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- An engineered streptavidin condensate platform for chemically inducible control of endogenous proteins in mammalian cells.bioRxiv : the preprint server for biology · 2026Article
- Spectrin condensates provide a nidus for assembling the axonal membrane-associated periodic skeleton.iScience · 2026Article
- Mechanosensitive dynamics of lysosomes along microtubules regulate leader cell emergence during collective cell migration.Nature communications · 2025Article
- Regulation of lipoprotein processing by GPNMB in foamy macrophages: potential therapeutic targets for atherosclerosis.Nature communications · 2025Article
- Chemical Control of Protease Activity and Gene Expression Using a Biotin-Released Inhibitory Domain.ACS synthetic biology · 2025Article
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- Matrix stiffening promotes perinuclear clustering of mitochondria.Molecular biology of the cell · 2024Article
- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Trafficking in cancer: from gene deregulation to altered organelles and emerging biophysical properties.Frontiers in cell and developmental biology · 2024Review
- Genetically encoded multimeric tags for subcellular protein localization in cryo-EM.Nature methods · 2023Article
- Condensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization.The EMBO journal · 2023Article
- Topology and function of translocated EspZ.mBio · 2023Article
- Microtubule retrograde flow retains neuronal polarization in a fluctuating state.Science advances · 2022Article
- RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.Nature communications · 2022Article
- Measurement of Lysosome Positioning by Shell Analysis and Line Scan.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.Cell reports · 2020Article
- Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.PLoS biology · 2020Article
- An optimized toolbox for the optogenetic control of intracellular transport.The Journal of cell biology · 2020Article
- A novel strategy to visualize vesicle-bound kinesins reveals the diversity of kinesin-mediated transport.Traffic (Copenhagen, Denmark) · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We report the development and characterization of a method, named reversible association with motor proteins (RAMP), for manipulation of organelle positioning within the cytoplasm. RAMP consists of coexpressing in cultured cells (i) an organellar protein fused to the streptavidin-binding peptide (SBP) and (ii) motor, neck, and coiled-coil domains from a plus-end-directed or minus-end-directed kinesin fused to streptavidin. The SBP-streptavidin interaction drives accumulation of organelles at the plus or minus end of microtubules, respectively. Importantly, competition of the streptavidin-SBP interaction by the addition of biotin to the culture medium rapidly dissociates the motor construct from the organelle, allowing restoration of normal patterns of organelle transport and distribution. A distinctive feature of this method is that organelles initially accumulate at either end of the microtubule network in the initial state and are subsequently released from this accumulation, allowing analyses of the movement of a synchronized population of organelles by endogenous motors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.