Evidence map›Paper›PMID 31098410›Full record

ArticleCommunications biology2019

Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.

Chieh-Han Lu, Wei-Chun Tang, Yen-Ting Liu, Shu-Wei Chang, Frances Camille M Wu, Chin-Yi Chen, Yun-Chi Tsai, Shun-Min Yang, Chiung-Wen Kuo, Yasushi Okada and 3 more

Abstract read
In one paragraph

Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

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  17. Plant-environment microscopy tracks interactions ofProceedings of the National Academy of Sciences of the United States of America · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Chieh-Han Lu *1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
Wei-Chun Tang *1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
Yen-Ting Liu *1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.ORCID 0000-0002-5768-0516
Shu-Wei Chang1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
Frances Camille M Wu1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.ORCID 0000-0002-8114-203X
Chin-Yi Chen1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
Yun-Chi Tsai1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.ORCID 0000-0002-3066-1235
Shun-Min Yang2Institute of Physics, Academia Sinica, Taipei, 11529 Taiwan.
Chiung-Wen Kuo1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
Yasushi Okada3Laboratory for Cell Polarity Regulation, Center for Biosystems Dynamics Research, RIKEN, Suita, Osaka, 565-0874 Japan.ORCID 0000-0003-2601-3689
Yeu-Kuang Hwu2Institute of Physics, Academia Sinica, Taipei, 11529 Taiwan.
Peilin Chen1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.ORCID 0000-0003-4154-0487
Bi-Chang Chen1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.ORCID 0000-0002-1876-7432

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent advances in super-resolution microscopy allow the localization of single molecules within individual cells but not within multiple whole cells due to weak signals from single molecules and slow acquisition process for point accumulation to reconstruct super-resolution images. Here, we report a fast, large-scale, and three-dimensional super-resolution fluorescence microscope based on single-wavelength Bessel lightsheet to selectively illuminate spontaneous blinking fluorophores tagged to the proteins of interest in space. Critical parameters such as labeling density, excitation power, and exposure time were systematically optimized resulting in a maximum imaging speed of 2.7 × 10

Indexed as

3T3 CellsAnimalsCells, CulturedFluorescent DyesImaging, Three-DimensionalMiceMicroscopy, FluorescenceNeuronsNuclear Pore Complex ProteinsRatsFluorescent DyesNuclear Pore Complex ProteinsNuclear envelopeSuper-resolution microscopy

Identifiers

PMID31098410
PMCPMC6509110

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.