ArticleIUCrJ2019
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.
Article in IUCrJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- LEGO®-inspired electrically-actuated microfluidics for on-chip protein crystallization andLab on a chip · 2026Article
- AXIS: a Lab-in-the-Loop machine learning approach for automated detection of macromolecular crystals.IUCrJ · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Protein crystallization and structure determination at room temperature in the CrystalChip.FEBS open bio · 2025Article
- Cyclic Olefin Copolymer-Based Fixed-Target Sample Delivery Device for Protein X-ray Crystallography.Analytical chemistry · 2024Article
- Practical courses on advanced methods in macromolecular crystallization: 20 years of history and future perspectives.Journal of applied crystallography · 2024Article
- Review
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- JINXED: just in time crystallization for easy structure determination of biological macromolecules.IUCrJ · 2023Article
- Polymer-based microfluidic device for on-chip counter-diffusive crystallization andLab on a chip · 2023Article
- Manufacturing of Ultra-Thin X-ray-Compatible COC Microfluidic Devices for Optimal In Situ Macromolecular Crystallography Experiments.Micromachines · 2022Article
- Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.Lab on a chip · 2021Article
- Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.International journal of molecular sciences · 2021Review
- Protein Crystallization in a Microfluidic Contactor with NafionMembranes · 2021Article
- Production of Cross-Linked Lipase Crystals at a Preparative Scale.Crystal growth & design · 2021Article
- Facile Fabrication of Protein-Macrocycle Frameworks.Journal of the American Chemical Society · 2021Article
- Discovery of Three Toxic Proteins ofViruses · 2020Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.