ArticleScientific reports2019
Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
77 citing papers in PubMed, 1 synthesis or guideline pooled it, 130 citations in OpenAlex.
- The impact of mitochondrial impairments on sperm function and male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2024Pooled it
- Comparative Efficacy of Microfluidics and Density Gradient Centrifugation for Sperm Preparation in IVF: A Randomized Controlled Trial.JBRA assisted reproduction · 2026Trial
- Microfluidic Intracytoplasmic Sperm Injection (MICSI): A Novel Platform for Sperm Isolation, Selection, and Injection Into Oocytes.Andrology · 2026Article
- Sperm DNA Fragmentation in Native Semen: A Reflection of Apoptotic and Non-Viable Spermatozoa and Its Implications for Assisted Reproduction.International journal of molecular sciences · 2026Article
- Sperm DNA fragmentation and its influence on mammalian reproduction.Nature reviews. Urology · 2026Review
- Automated oocyte retrieval, denudation, sperm preparation, and ICSI in the IVF laboratory: a proof-of-concept study and report of the first live births.Human reproduction (Oxford, England) · 2026Article
- Viable sperm DNA fragmentation index (SDF) provides better diagnostic accuracy than total SDF in male infertility: a cross-sectional study.Translational andrology and urology · 2026Article
- Protective Effect ofAntioxidants (Basel, Switzerland) · 2026Article
- Sperm DNA Fragmentation: The Concept and Its Use in Assisted Reproduction.Advances in experimental medicine and biology · 2026Review
- Association of sperm DNA fragmentation with abstinence duration, seminal oxidative stress and age: a call to consider abstinence in andrological diagnostics.Frontiers in urology · 2026Article
- High sperm DNA fragmentation is associated with increased embryonic chromosomal abnormality: a retrospective study of 8 years involving 5637 couples undergoing ART.Human reproduction open · 2026Article
- Article
- Review
- Effect of hepatitis B virus infection in males on pregnancy outcomes of intrauterine insemination.Scientific reports · 2025Article
- Sperm preparation techniques affect biological parameters, fertility indices, and DNA fragmentation in patients with oligo-astheno-teratozoospermia.Reproduction & fertility · 2025Article
- Efficient methods of isolation and purification of extracellular vesicles.Nano convergence · 2025Review
- Article
- Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis.ACS nano · 2025Review
- Hurdles of Sperm Success: Exploring the Role of DNases.International journal of molecular sciences · 2025Review
- Simple and rapid microfluidic device for processing raw human semen and separation of motile sperm based on their intrinsic behavior and morphology.Scientific reports · 2025Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common sperm selection procedure in Assisted Reproduction Techniques (ARTs), experience a 50% lower probability of pregnancy. Hence, identification of these subjects is of clinical importance. Here, we investigated whether such subjects are identified with higher accuracy detecting DNA fragmentation in viable (viable sDF) instead of total spermatozoa (total sDF) and whether swim up, an alternative procedure to DGC, does not increase sDF. With DGC, we identified 10/20 subjects increasing total sDF, and 2 more subjects using viable sDF. With swim up, we identified 8/40 subjects increasing total sDF, and 8 more subjects using viable sDF. In addition, viable sDF reveals more accurately the increase of the damage when it occurs. Finally, a multivariate analysis demonstrated that the proportional increase of sDF was higher after DGC respect to swim up. In conclusion, viable sDF is a more accurate parameter to reveal the increase of the damage by selection both with swim up and DGC. Swim up increases sDF in some samples, although at a lesser extent than DGC, suggesting that it should be used to select spermatozoa for ARTs when possible.
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Registered trials
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