ArticleParasites & vectors2019
Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 53 citations in OpenAlex.
- Angiostrongylus cantonensis Rat Lungworm Detected in Rats, Madagascar, 2022-2023.Emerging infectious diseases · 2026Article
- Sensitive Detection of Foodborne Parasitic Larvae Using a Sieving-qPCR Workflow: Application to Angiostrongylus cantonensis.Journal of food science · 2026Article
- Hedgehogs and Angiostrongylus cantonensis: Uncovering the Role of Atelerix albiventris in the Parasite Life Cycle.Integrative zoology · 2026Article
- New tools to uncover old tricks: an update on the knowledge on the most successful invasive freshwater helminth, Schyzocotyle acheilognathi.Parasitology research · 2025Review
- Evidence of new endemic foci of the foodborne helminths Angiostrongylus spp. in rats in selected communities in the Philippines.Parasites & vectors · 2025Article
- Same parasite, diverging fates: distinct responses of tenrecs to Angiostrongylus cantonensis infection.Veterinary research communications · 2025Article
- First record ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- Isolate-specific rat brain transcriptional responses to rat lungworm (Angiostrongylus cantonensis).Pathogens and disease · 2025Article
- Passage ofParasitology · 2025Article
- Analysis of the mitochondrial genome to determine the origins and pathways of entry ofParasitology · 2024Article
- Insights into the genetic diversity ofFood and waterborne parasitology · 2024Review
- Release of Angiostrongylus cantonensis larvae from live intermediate hosts under stress.Parasitology research · 2024Article
- The ZoonoticPathogens (Basel, Switzerland) · 2024Article
- Older urban rats are infected with the zoonotic nematodeCurrent research in parasitology & vector-borne diseases · 2024Article
- Fatal neural angiostrongyliasis in the Bolivian squirrel monkey (One health (Amsterdam, Netherlands) · 2023Article
- Rat lungworm survives winter: experimental overwintering ofParasitology · 2023Article
- The recent introduction of Angiostrongylus cantonensis and its intermediate host Achatina fulica into Guadeloupe detected by phylogenetic analyses.Parasites & vectors · 2023Article
- Immunodiagnostic Detection of Angiostrongylus cantonensis Exposure on Hawaii Island Using Isogeographic 31-kDa Antigen.The American journal of tropical medicine and hygiene · 2023Article
- Rat lungworm (Parasitology · 2023Article
- In the Dawn of an Early Invasion: No Genetic Diversity ofPathogens (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 6 countries.
Funding
Abstract
backgroundRats (Rattus spp.) invaded most of the world as stowaways including some that carried the rat lungworm, Angiostrongylus cantonensis, the cause of eosinophilic meningoencephalitis in humans and other warm-blooded animals. A high genetic diversity of A. cantonensis based on short mitochondrial DNA regions is reported from Southeast Asia. However, the identity of invasive A. cantonensis is known for only a minority of countries. The affordability of next-generation sequencing for characterisation of A. cantonensis genomes should enable new insights into rat lung worm invasion and parasite identification in experimental studies.
methodsGenomic DNA from morphologically verified A. cantonensis (two laboratory-maintained strains and two field isolates) was sequenced using low coverage whole genome sequencing. The complete mitochondrial genome was assembled and compared to published A. cantonensis and Angiostrongylus malaysiensis sequences. To determine if the commonly sequenced partial cox1 can unequivocally identify A. cantonensis genetic lineages, the diversity of cox1 was re-evaluated in the context of the publicly available cox1 sequences and the entire mitochondrial genomes. Published experimental studies available in Web of Science were systematically reviewed to reveal published identities of A. cantonensis used in experimental studies.
resultsNew A. cantonensis mitochondrial genomes from Sydney (Australia), Hawaii (USA), Canary Islands (Spain) and Fatu Hiva (French Polynesia), were assembled from next-generation sequencing data. Comparison of A. cantonensis mitochondrial genomes from outside of Southeast Asia showed low genetic diversity (0.02-1.03%) within a single lineage of A. cantonensis. Both cox1 and cox2 were considered the preferred markers for A. cantonensis haplotype identification. Systematic review revealed that unequivocal A. cantonensis identification of strains used in experimental studies is hindered by absence of their genetic and geographical identity.
conclusionsLow coverage whole genome sequencing provides data enabling standardised identification of A. cantonensis laboratory strains and field isolates. The phenotype of invasive A. cantonensis, such as the capacity to establish in new territories, has a strong genetic component, as the A. cantonensis found outside of the original endemic area are genetically uniform. It is imperative that the genotype of A. cantonensis strains maintained in laboratories and used in experimental studies is unequivocally characterised.
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