ArticleeLife2019
Asymmetry between the two acidic patches dictates the direction of nucleosome sliding by the ISWI chromatin remodeler.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed.
- DNA-PK driven nucleosome unwrapping enables NHEJ in chromatin.Nature communications · 2026Article
- Native nucleosome-positioning elements as alternatives to the 601 sequence for nucleosome repositioning studies.Nucleic acids research · 2025Article
- A competitive regulatory mechanism of the Chd1 remodeler is integral to distorting nucleosomal DNA.Nature structural & molecular biology · 2025Article
- Structural basis of human CHD1 nucleosome recruitment and pausing.Molecular cell · 2025Article
- Conformational switching of Arp5 subunit regulates INO80 chromatin remodeling.Nucleic acids research · 2025Article
- H3K56 acetylation regulates chromatin maturation following DNA replication.Nature communications · 2025Article
- Structural basis for linker histone H5-nucleosome binding and chromatin fiber compaction.Cell research · 2024Article
- Dual engagement of the nucleosomal acidic patches is essential for deposition of histone H2A.Z by SWR1C.eLife · 2024Article
- Conformational switching of Arp5 subunit differentially regulates INO80 chromatin remodeling.bioRxiv : the preprint server for biology · 2024Article
- Bi-directional nucleosome sliding by the Chd1 chromatin remodeler integrates intrinsic sequence-dependent and ATP-dependent nucleosome positioning.Nucleic acids research · 2023Article
- Reorientation of INO80 on hexasomes reveals basis for mechanistic versatility.Science (New York, N.Y.) · 2023Article
- Human SMARCA5 is continuously required to maintain nucleosome spacing.Molecular cell · 2023Article
- Oncohistones: Exposing the nuances and vulnerabilities of epigenetic regulation.Molecular cell · 2022Review
- In Vitro Mapping of Nucleosome Positions at Base-Pair Resolution Using Ortho-Phenanthroline.Current protocols · 2022Article
- A hexasome is the preferred substrate for the INO80 chromatin remodeling complex, allowing versatility of function.Molecular cell · 2022Article
- A Genetically Encoded Approach for Breaking Chromatin Symmetry.ACS central science · 2022Article
- Synthesis of Oriented Hexasomes and Asymmetric Nucleosomes Using a Template Editing Process.Journal of the American Chemical Society · 2022Article
- Janus Bioparticles: Asymmetric Nucleosomes and Their Preparation Using Chemical Biology Approaches.Accounts of chemical research · 2021Review
- Sophisticated Conversations between Chromatin and Chromatin Remodelers, and Dissonances in Cancer.International journal of molecular sciences · 2021Review
- Biophysics of Chromatin Remodeling.Annual review of biophysics · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The acidic patch is a functionally important epitope on each face of the nucleosome that affects chromatin remodeling. Although related by 2-fold symmetry of the nucleosome, each acidic patch is uniquely positioned relative to a bound remodeler. An open question is whether remodelers are distinctly responsive to each acidic patch. Previously we reported a method for homogeneously producing asymmetric nucleosomes with distinct H2A/H2B dimers (Levendosky et al., 2016). Here, we use this methodology to show that the Chd1 remodeler from Saccharomyces cerevisiae and ISWI remodelers from human and Drosophila have distinct spatial requirements for the acidic patch. Unlike Chd1, which is equally affected by entry- and exit-side mutations, ISWI remodelers strongly depend on the entry-side acidic patch. Remarkably, asymmetry in the two acidic patches stimulates ISWI to slide mononucleosomes off DNA ends, overriding the remodeler's preference to shift the histone core toward longer flanking DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.