ArticleJournal of nematology2018
Improved 18S small subunit rDNA primers for problematic nematode amplification.
Article in Journal of nematology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Living Environment and Basic Features of the Nematodes Associated with Dung BeetleJournal of nematology · 2025Article
- Identification of potential insect ecological interactions using a metabarcoding approach.PeerJ · 2025Article
- Parasitism and branchitis in various fish species from 4 Cook County, Illinois inland freshwater lakes, 5-year survey, 2017-2021.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- First Report and Molecular Variability ofJournal of nematology · 2024Article
- Geographical distribution and phoretic associations of the viviparous nematodeJournal of nematology · 2024Article
- Current advances in the identification of plant nematode diseases: From lab assays to in-field diagnostics.Frontiers in plant science · 2023Review
- Article
- Observation onHelminthologia · 2022Article
- Morphometric and Molecular Diversity among Seven European Isolates ofPlants (Basel, Switzerland) · 2021Article
- Species Diversity of Pin Nematodes (Plants (Basel, Switzerland) · 2021Article
- Characterization ofJournal of nematology · 2020Article
- Improvement of long segment ribosomal PCR amplification for molecular identification ofJournal of nematology · 2020Article
- PCR amplification of a long rDNA segment with one primer pair in agriculturally important nematodes.Journal of nematology · 2019Article
- First report ofJournal of nematology · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new primers were developed based on a very large 276 taxon alignment of 124 agriculturally important nematode species, and tested on problematic nematode taxa such as The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new primers were developed based on a very large 276 taxon alignment of 124 agriculturally important nematode species, and tested on problematic nematode taxa such as
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