ArticleMolecular biology reports2019
Optimized Ki-67 staining in murine cells: a tool to determine cell proliferation.
Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Pancreatic Tissue Remodeling and Fibrosis After Irreversible Electroporation: A Histopathological and Thermal Perspective.Biomedicines · 2025Article
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- Transcriptomic dynamics and cell-to-cell communication during the transition of prospermatogonia to spermatogonia revealed at single-cell resolution.BMC genomics · 2025Article
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- MYC Inhibition Potentiates CD8+ T Cells Against Multiple Myeloma and Overcomes Immunomodulatory Drug Resistance.Clinical cancer research : an official journal of the American Association for Cancer Research · 2024Article
- Immunohistochemical Expression Levels of Epidermal Growth Factor Receptor, Cyclooxygenase-2, and Ki-67 in Canine Cutaneous Squamous Cell Carcinomas.Current issues in molecular biology · 2024Article
- Improved Preservation of Mouse Intestinal Tissue Using a Formalin/Acetic Acid Fixative and Quantitative Histological Analysis Using QuPath.Current protocols · 2024Article
- 3D culture of alginate-hyaluronic acid hydrogel supports the stemness of human mesenchymal stem cells.Scientific reports · 2024Article
- Epidermal stem cell-derived exosomes improve wound healing by promoting the proliferation and migration of human skin fibroblasts.Burns & trauma · 2024Article
- Differentiation ability of hematopoietic stem cells and mesenchymal stem cells isolated from human peripheral blood.Frontiers in cell and developmental biology · 2024Article
- Oxygen-glucose deprivation-induced glial cell reactivity in the rat primary neuron-glia co-culture.The Journal of veterinary medical science · 2023Article
- Article
- Unraveling astrocyte behavior in the space brain: Radiation response of primary astrocytes.Frontiers in public health · 2023Article
- Polycationic peptide RBiochemistry and biophysics reports · 2022Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
The reliable analysis of the cell cycle status has become increasingly relevant for scientific and clinical work, especially for the determination of tumor cell growth. One established method to characterize the proliferation activity of cells is the analysis of the Ki-67 protein. Ki-67 is expressed in the nucleus during the whole cell cycle except for the G0 phase. Several different protocols exist for the examination of the Ki-67 protein in tissue and cell culture, but most of them are defined for human cells. For the analysis of the Ki-67 protein in murine tissue and cell culture there is a variety of protocols existing which recommend different fixation and permeabilization reagents or special kits. In this study, we established a reliable protocol for Ki-67 staining in murine cells and tissue based on PFA fixation, which can be used not only for flow cytometry but also for immunofluorescence microscopy analysis. We tested our protocol successfully with three different Ki-67 anti-mouse antibodies in cell culture, regenerating liver tissue and mouse melanoma tumor to demonstrate the general applicability.
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Registered trials
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