ArticleMarine biotechnology (New York, N.Y.)2019
RNA Interference by Ingested dsRNA-Expressing Bacteria to Study Shell Biosynthesis and Pigmentation in Crassostrea gigas.
Article in Marine biotechnology (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 53 citations in OpenAlex.
- A methodology for RNAi gene knockdown in the marine annelid Streblospio benedicti by delivering dsRNA.Development (Cambridge, England) · 2026Article
- Implications of Endogenous Small Regulatory RNAs Survey in Mollusks on Gene Silencing Approaches.Marine biotechnology (New York, N.Y.) · 2025Article
- Somatostatin Receptor Gene Functions in Growth Regulation in Bivalve Scallop and Clam.International journal of molecular sciences · 2024Article
- Review
- Multi-omic insights into the formation and evolution of a novel shell microstructure in oysters.BMC biology · 2023Article
- Heme-Peroxidase 2 Modulated by POU2F1 and SOX5 is Involved in Pigmentation in Pacific Oyster (Crassostrea gigas).Marine biotechnology (New York, N.Y.) · 2022Article
- The Role of Anti-Viral Effector Molecules in Mollusc Hemolymph.Biomolecules · 2022Review
- Shell Biosynthesis and Pigmentation as Revealed by the Expression of Tyrosinase and Tyrosinase-like Protein Genes in Pacific Oyster (Crassostrea gigas) with Different Shell Colors.Marine biotechnology (New York, N.Y.) · 2021Article
- RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation inInternational journal of molecular sciences · 2021Article
- Integrated analysis of microRNA and mRNA expression profiles in Crassostrea gigas to reveal functional miRNA and miRNA-targets regulating shell pigmentation.Scientific reports · 2020Article
- Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla-Towards Finding the Missing Link in Achelata.International journal of molecular sciences · 2020Article
- Abalone Viral Ganglioneuritis.Pathogens (Basel, Switzerland) · 2020Review
- siRNA-Mediated MrIAG Silencing Induces Sex Reversal in Macrobrachium rosenbergii.Marine biotechnology (New York, N.Y.) · 2020Article
- PfSMAD1/5 Can Interact with PfSMAD4 to Inhibit PfMSX to Regulate Shell Biomineralization in Pinctada fucata martensii.Marine biotechnology (New York, N.Y.) · 2020Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
RNA interference (RNAi) is an important molecular tool for analysis of gene function in vivo. Although the Pacific oyster Crassostrea gigas is an economically important species with fully sequenced genome, very few mechanistic studies have been carried out due to the lack of molecular techniques to alter gene expression without inducing stress. In this present study, we used unicellular alga Platymonas subcordiformis and Nitzschia closterium f. minutissima as a vector to feed oysters with Escherichia coli strain HT115 engineered to express double-stranded RNAs (dsRNAs) targeting specific genes involved in shell pigmentation. A C. gigas strain with black shell was used to target tyrosinase or peroxidase gene expression by RNAi using the above-mentioned approach. The results showed that feeding oyster with dsRNA of tyrosinase could knock down the expression of corresponding tyrosinase and hinder the developed shell growth. Feeding oyster with dsRNA of peroxidase could knock down the expression of the corresponding peroxidase and result in reduced black pigmentation in the newly developed shell. This non-invasive RNAi study demonstrated that tyrosinase played a vital role in the assembly and maturation of shell matrices and peroxidase was essential for black pigmentation in the shell. Moreover, the RNA interference by ingested dsRNA-expressing bacteria is a relatively simple and effective method for knockdown of a gene expression in adult oysters, thus further advances the use of C. gigas as model organism in functional genomic studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.