ArticleScience advances2019
Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Label-Free and Immobilization-Free Protein-Binding Assays by Ultraviolet Transient Absorption Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Color-resolved third harmonic generation microscopy for single-RBC HbA1c measurement and glycemic history assessment.Science advances · 2025Article
- Development and validation of a nomogram of all-cause mortality in adult Americans with diabetes.Scientific reports · 2024Article
- Emerging biosensor probes for glycated hemoglobin (HbA1c) detection.Mikrochimica acta · 2024Review
- Progress and Prospects in Optical Ultrafast Microscopy in the Visible Spectral Region: Transient Absorption and Two-Dimensional Microscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023Review
- The associations between dietary flavonoid intake and the prevalence of diabetes mellitus: Data from the National Health and Nutrition Examination Survey 2007-2010 and 2017-2018.Frontiers in endocrinology · 2023Article
- Association Between Visceral Fat Area and Glycated Hemoglobin in Type 2 Diabetics: A Retrospective Study.Diabetes, metabolic syndrome and obesity : targets and therapy · 2023Article
- Photoinactivation of catalase sensitizes a wide range of bacteria to ROS-producing agents and immune cells.JCI insight · 2022Article
- Transient absorption spectroscopy and imaging of redox in muscle mitochondria.Biomedical optics express · 2022Article
- In vivo simultaneous nonlinear absorption Raman and fluorescence (SNARF) imaging of mouse brain cortical structures.Communications biology · 2022Article
- Transient absorption microscopy: Technological innovations and applications in materials science and life science.The Journal of chemical physics · 2020Article
- Quantitative imaging of intraerythrocytic hemozoin by transient absorption microscopy.Journal of biomedical optics · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
As a stable and accurate biomarker, glycated hemoglobin (HbA1c) is clinically used to diagnose diabetes with a threshold of 6.5% among total hemoglobin (Hb). Current methods such as boronate affinity chromatography involve complex processing of large-volume blood samples. Moreover, these methods cannot measure HbA1c fraction at single-red blood cell (RBC) level, thus unable to separate the contribution from other factors such as RBC lifetime. Here, we demonstrate a spectroscopic transient absorption imaging approach that is able to differentiate HbA1c from Hb on the basis of their distinct excited-state dynamics. HbA1c fraction inside a single RBC is derived quantitatively through phasor analysis. HbA1c fraction distribution of diabetic blood is apparently different from that of healthy blood. A mathematical model is developed to derive the long-term blood glucose concentration. Our technology provides a unique way to study heme modification and to derive clinically important information void of bloodstream glucose fluctuation.
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