Evidence map›Paper›PMID 31091699›Full record

ArticleInternational journal of molecular sciences2019

Renal Regenerative Potential of Extracellular Vesicles Derived from miRNA-Engineered Mesenchymal Stromal Cells.

Marta Tapparo, Stefania Bruno, Federica Collino, Gabriele Togliatto, Maria Chiara Deregibus, Paolo Provero, Sicheng Wen, Peter J Quesenberry, Giovanni Camussi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 2 syntheses or guidelines pooled it, 63 citations in OpenAlex.

  1. Pooled it
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  12. Bone-organ axes: bidirectional crosstalk.Military Medical Research · 2024
    Review
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  14. Article
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  16. Article
  17. Review
  18. Extracellular Vesicle MicroRNA in the Kidney.Comprehensive Physiology · 2023
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Marta TapparoDepartment of Medical Sciences and Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy. marta.tapparo@unito.it.
Stefania BrunoDepartment of Medical Sciences and Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy. stefania.bruno@unito.it.
Federica CollinoDepartment of Biomedical Sciences and Paediatric Research Institute "Citta della Speranza", University of Padova, 35129 Padova, Italy. federica.collino@unipd.it.ORCID 0000-0002-3619-7701
Gabriele TogliattoDepartment of Medical Sciences and Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy. gabriele.togliatto@unito.it.
Maria Chiara Deregibus2i3T Società per la gestione dell'incubatore di imprese e per il trasferimento tecnologico Scarl, University of Torino, 10126 Torino, Italy. mariachiara.deregibus@unito.it.
Paolo ProveroDepartment of Molecular Biotechnology and Health Sciences and Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy. paolo.provero@unito.it.
Sicheng WenDivision of Hematology/Oncology, Brown University, Rhode Island Hospital, Providence, Rhode Island, RI 02912, USA. swen@lifespan.org.
Peter J QuesenberryDivision of Hematology/Oncology, Brown University, Rhode Island Hospital, Providence, Rhode Island, RI 02912, USA. PQuesenberry@lifespan.org.
Giovanni CamussiDepartment of Medical Sciences and Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy. giovanni.camussi@unito.it.
University of Turin · ITBrown University · USCittà della Speranza Foundation · IT

Funding

Office of Extramural Research, National Institutes of Health grants UH2-TR000880, UH3TR000880-03S1
6 · The paper itself

Abstract

Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) possess pro-regenerative potential in different animal models with renal injury. EVs contain different molecules, including proteins, lipids and nucleic acids. Among the shuttled molecules, miRNAs have a relevant role in the pro-regenerative effects of EVs and are a promising target for therapeutic interventions. The aim of this study was to increase the content of specific miRNAs in EVs that are known to be involved in the pro-regenerative effect of EVs, and to assess the capacity of modified EVs to contribute to renal regeneration in in vivo models with acute kidney injuries. To this purpose, MSCs were transiently transfected with specific miRNA mimics by electroporation. Molecular analyses showed that, after transfection, MSCs and derived EVs were efficiently enriched in the selected miRNAs. In vitro and in vivo experiments indicated that EVs engineered with miRNAs maintained their pro-regenerative effects. Of relevance, engineered EVs were more effective than EVs derived from naïve MSCs when used at suboptimal doses. This suggests the potential use of a low amount of EVs (82.5 × 10

Indexed as

RegenerationAcute Kidney InjuryAnimalsCells, CulturedExtracellular VesiclesHumansMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMiceMice, SCIDMicroRNAsRNAi TherapeuticsMicroRNAsacute kidney injuryextracellular vesiclesmesenchymal stromal cellsmicroRNAmodified-MSCs

Identifiers

PMID31091699
PMCPMC6567878
OpenAlexW2946589464

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.