ArticleCell reports2019
Two-Color Volumetric Imaging of Neuronal Activity of Cortical Columns.
Article in Cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Simultaneous single-cell calcium imaging of neuronal population activity and brain-wide BOLD fMRI.Nature methods · 2026Article
- Synaptic Development of Fine Spatial Scale Organization of Neuronal Orientation Tuning in Mouse Primary Visual Cortex.bioRxiv : the preprint server for biology · 2026Article
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Needle beam two-photon microscopy for simultaneous multiplane neural and vascular imaging in awake mice.PhotoniX · 2026Article
- High-speed neural imaging with multiplexed miniaturized two-photon microscopy.Cell reports methods · 2025Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Circuit-Based Understanding of Fine Spatial Scale Clustering of Orientation Tuning in Mouse Visual Cortex.bioRxiv : the preprint server for biology · 2025Article
- Window into the Brain: In Vivo Multiphoton Imaging.ACS photonics · 2025Review
- An end-to-end recurrent compressed sensing method to denoise, detect and demix calcium imaging data.Nature machine intelligence · 2024Article
- Article
- Behavior-relevant top-down cross-modal predictions in mouse neocortex.Nature neuroscience · 2024Article
- Dual-resonant scanning multiphoton microscope with ultrasound lens and resonant mirror for rapid volumetric imaging.Scientific reports · 2023Article
- Practical considerations in an era of multicolor optogenetics.Frontiers in cellular neuroscience · 2023Article
- Learning cortical hierarchies with temporal Hebbian updates.Frontiers in computational neuroscience · 2023Article
- Three-dimensional-generator U-net for dual-resonant scanning multiphoton microscopy image inpainting and denoising.Biomedical optics express · 2022Article
- Characterization of red fluorescent reporters for dual-colorNeurophotonics · 2022Article
- Review
- Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.Nature communications · 2022Article
- Manipulating neuronal circuits, in concert.Science (New York, N.Y.) · 2021Article
- Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice.Journal of visualized experiments : JoVE · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activity at cellular resolution is needed. Here, we introduce wavelength multiplexing to perform fast volumetric two-photon imaging of cortical columns (>2,000 neurons in 10 planes at 10 vol/s), using two different calcium indicators, an electrically tunable lens and a spatial light modulator. We image the activity of neuronal populations from layers 2/3 to 5 of primary visual cortex from awake mice, finding a lack of columnar structures in orientation responses and revealing correlations between layers which differ from trial to trial. We also simultaneously image functional correlations between presynaptic layer 1 axons and postsynaptic layer 2/3 neurons. Wavelength multiplexing enhances high-speed volumetric microscopy and can be combined with other optical multiplexing methods to easily boost imaging throughput.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.