ArticlePloS one2019
A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- Molecular Insights into the Activation of a Fungal Copper Radical Oxidase by Peroxidases.Chem & bio engineering · 2026Article
- Expansion of Auxiliary Activity Family 5 sequence space via biochemical characterization of six new copper radical oxidases.Applied and environmental microbiology · 2024Article
- Enzymatically Oxidized Carbohydrates As Dicarbonyl Biobased Cross-Linkers for Polyamines.Biomacromolecules · 2024Article
- Copper-radical oxidases: A diverse group of biocatalysts with distinct properties and a broad range of biotechnological applications.Engineering microbiology · 2022Review
- Active-Site Engineering Switches Carbohydrate Regiospecificity in a Fungal Copper Radical Oxidase.ACS catalysis · 2022Article
- A survey of substrate specificity among Auxiliary Activity Family 5 copper radical oxidases.Cellular and molecular life sciences : CMLS · 2021Article
- A Putative Lignin Copper Oxidase fromJournal of fungi (Basel, Switzerland) · 2021Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.
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