Evidence map›Paper›PMID 31091283›Full record

ArticlePloS one2019

Recovery of influenza A viruses from lake water and sediments by experimental inoculation.

Daniela Numberger, Carola Dreier, Colin Vullioud, Gülsah Gabriel, Alex D Greenwood, Hans-Peter Grossart

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Daniela NumbergerLeibniz Institute for Zoo and Wildlife Research, Berlin, Germany.ORCID 0000-0002-0029-231X
Carola DreierHeinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
Colin VullioudLeibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
Gülsah GabrielHeinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
Alex D GreenwoodLeibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
Hans-Peter GrossartUniversity of Potsdam, Institute of Biochemistry and Biology, Potsdam, Germany.
Leibniz Institute for Zoo and Wildlife Research · DELeibniz Institute of Virology (LIV) · DELeibniz Institute of Freshwater Ecology and Inland Fisheries · DEUniversity of Potsdam · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Influenza A viruses (IAV) are zoonotic pathogens relevant to human, domestic animal and wildlife health. Many avian IAVs are transmitted among waterfowl via a faecal-oral-route. Therefore, environmental water where waterfowl congregate may play an important role in the ecology and epidemiology of avian IAV. Water and sediment may sustain and transmit virus among individuals or species. It is unclear at what concentrations waterborne viruses are infectious or remain detectable. To address this, we performed lake water and sediment dilution experiments with varying concentrations or infectious doses of four IAV strains from seal, turkey, duck and gull. To test for infectivity of the IAV strains in a concentration dependent manner, we applied cultivation to specific pathogen free (SPF) embryonated chicken eggs and Madin-Darby Canine Kidney (MDCK) cells. IAV recovery was more effective from embryonated chicken eggs than MDCK cells for freshwater lake dilutions, whereas, MDCK cells were more effective for viral recovery from sediment samples. Low infectious dose (1 PFU/200 μL) was sufficient in most cases to detect and recover IAV from lake water dilutions. Sediment required higher initial infectious doses (≥ 100 PFU/200 μL).

Indexed as

Microbial ViabilityWater MicrobiologyAnimalsChick EmbryoDogsGeologic SedimentsInfluenza A virusLakesMadin Darby Canine Kidney CellsWaterWater

Identifiers

PMID31091283
PMCPMC6519785
OpenAlexW2946758816

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.