ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2019
Tissue Morphology and Antigenicity in Mouse and Rat Tibia: Comparing 12 Different Decalcification Conditions.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- The coccosphere of the heavy calcifying coccolithophore Coccolithus braarudii provides defense against bacteria.Communications biology · 2026Article
- Metformin promotes healthspan by attenuating frailty and preserving musculoskeletal function-structure in middle-aged mice.Biogerontology · 2026Article
- Intra-Articular N-Acetylcysteine Reduces Synovitis Without Preventing Cartilage Degeneration in Experimental Osteoarthritis.Biomedicines · 2025Article
- Identification of Optimal Decalcification Method and Tissue Preparation Protocol for RNAscope In Situ Hybridization in Rodent Incisor Tooth.Dentistry journal · 2025Article
- Recent Advances in Experimental Functional Characterization of GWAS Candidate Genes in Osteoporosis.International journal of molecular sciences · 2025Review
- High-fat diet-induced osteoporosis in mice under hypoxic conditions.BMC musculoskeletal disorders · 2025Article
- Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis.Scientific reports · 2025Article
- Article
- Hypertonic saline- and detergent-accelerated EDTA-based decalcification better preserves mRNA of bones.Scientific reports · 2024Article
- RIO-kinase 2 is essential for hematopoiesis.PloS one · 2024Article
- Optimization of the optical transparency of bones by PACT-based passive tissue clearing.Experimental & molecular medicine · 2023Article
- Modified Periodic Acid-Schiff (PAS) Is an Alternative to Safranin O for Discriminating Bone-Cartilage Interfaces.JBMR plus · 2023Article
- Arduino Automated Microwave Oven for Tissue Decalcification.Bioengineering (Basel, Switzerland) · 2023Article
- Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis.Frontiers in bioengineering and biotechnology · 2023Article
- Multicolor Histochemical Staining for Identification of Mineralized and Non-Mineralized Musculoskeletal Tissue: Immunohistochemical and Radiological Validation in Decalcified Bone Samples.Bioengineering (Basel, Switzerland) · 2022Article
- Novel polychrome staining distinguishing osteochondral tissue and bone cells in decalcified paraffin sections.Cell and tissue research · 2021Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional bone decalcification is a time-consuming process and is therefore unsuitable for clinical applications and time-limited research projects. Consequently, we compared the effect of four different decalcification solutions applied at three different temperatures, and assessed the rate of decalcification and the implications on tissue morphology and antigenicity of mouse and rat tibiae. Bones were decalcified with 10% ethylenediaminetetraacetic acid (EDTA), 10% formic acid, 5% hydrochloric acid, and 5% nitric acid at 4C, 25C, and 37C. Decalcification in both species was fastest in nitric acid at 37C and slowest in EDTA at 4C. Histological and immunohistochemical staining confirmed that the conventional protocols of EDTA at 4C and 25C remain the best option regarding the quality of tissue preservation. Whereas formic acid at 4C is a good alternative saving about 90% of the decalcification time, hydrochloric and nitric acids should be avoided particularly in case of rat tibia. By contrast, due to their smaller size, mouse tibiae had shorter decalcification times and tolerated higher temperatures and exposure to acids much better. In conclusion, this study demonstrated that depending on the specific research question and sample size, alternative decalcification methods could be used to decrease the time of decalcification while maintaining histological accuracy.
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