ArticleMolecular biology reports2019
Human sperm motility: a molecular study of mitochondrial DNA, mitochondrial transcription factor A gene and DNA fragmentation.
Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Beyond semen analysis: in men with normal semen parameters telomere attrition and oxidative imbalance distinguish those fertile from those with infertility.Journal of translational medicine · 2026Article
- The RNA helicase DDX53 (CAGE) contributes to RNA metabolism in a human germ cell model.bioRxiv : the preprint server for biology · 2026Article
- Regulation of mitochondrial function during spermatogenesis and sperm maturation.Cellular & molecular biology letters · 2026Review
- Sperm mtDNA Copy Number Is Not Associated With Midpiece Size Among Songbirds.Ecology and evolution · 2025Article
- Relation between proteome characterization and semen quality in Italian chicken breeds.PloS one · 2025Article
- Deciphering the role of reactive oxygen species in idiopathic asthenozoospermia.Frontiers in endocrinology · 2025Review
- Mitochondrial Morphology and Function Abnormality in Ovarian Granulosa Cells of Patients with Diminished Ovarian Reserve.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Review
- Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs.Journal of animal science and biotechnology · 2024Article
- Copy number variations (CNVs) and karyotyping analysis in males with azoospermia and oligospermia.BMC medical genomics · 2023Article
- Mitochondria Quality Control and Male Fertility.Biology · 2023Review
- Testosterone Serum Levels Are Related to Sperm DNA Fragmentation Index Reduction after FSH Administration in Males with Idiopathic Infertility.Biomedicines · 2022Article
- Assessment of the Role of Nuclear ENDOG Gene and mtDNA Variations on Paternal Mitochondrial Elimination (PME) in Infertile Men: An Experimental Study.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Green Tea Extract in the Extender Improved the Post-Thawed Semen Quality and Decreased Amino Acid Mutation of Kacang Buck Sperm.Veterinary sciences · 2022Article
- Article
- Unraveling the Molecular Impact of Sperm DNA Damage on Human Reproduction.Advances in experimental medicine and biology · 2022Article
- Spermatozoa Develop Molecular Machinery to Recover From Acute Stress.Frontiers in endocrinology · 2022Article
- Associations of Sperm mtDNA Copy Number, DNA Fragmentation Index, and Reactive Oxygen Species With Clinical Outcomes in ART Treatments.Frontiers in endocrinology · 2022Article
- Mitochondrial Dynamics Markers and Related Signaling Molecules Are Important Regulators of Spermatozoa Number and Functionality.International journal of molecular sciences · 2021Article
- Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm.Heliyon · 2021Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Alterations affecting the mitochondrial genome and chromatin integrity of spermatozoa impair male reproductive potential. This study aimed to evaluate the impact of mitochondrial DNA (mtDNA) copy number alterations on sperm motility and on the molecular mechanism regulating the number of mtDNA copies, through analysis of mitochondrial transcription factor A (TFAM) gene expression. It also investigated any correlation between mtDNA copy number and sperm DNA fragmentation (SDF). Sixty-three asthenozoospermic semen samples (Group A) and 63 normokinetic semen samples (Group N) were analysed according to WHO (WHO laboratory manual for the examination and processing of human semen, World Health Organization, Geneva, 2010). Sperm mtDNA copy number and TFAM gene expression were quantified by real time quantitative polymerase chain reaction. SDF was evaluated using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay. The mtDNA copy number was higher in asthenozoospermic semen samples and was negatively correlated with sperm concentration, total sperm number and total motile spermatozoa. The caseload showed a global negative correlation of TFAM gene expression with total motile sperm and a positive correlation with abnormal forms, SDF and mtDNA copy number, but this was not confirmed within each subgroup. SDF was significantly increased in asthenozoospermic samples and correlated with abnormal forms. No correlation was found between SDF and mtDNA copy number. Our results suggest a potential role of mtDNA content as an indicator of semen quality and support the hypothesis that dysregulation of TFAM expression is accompanied by a qualitative impairment of spermatogenesis. Since mtDNA copy number alterations and impaired chromatin integrity could affect reproductive success, these aspects should be evaluated in relation to assisted reproductive techniques.
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