ArticleFEMS microbiology letters2019
Unexpected connections between type VI-B CRISPR-Cas systems, bacterial natural competence, ubiquitin signaling network and DNA modification through a distinct family of membrane proteins.
Article in FEMS microbiology letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Comprehensive analysis of CRISPR array repeat mutations reveals subtype-specific patterns and links to spacer dynamics.microLife · 2026Article
- Characterization of Five CRISPR Systems inInternational journal of molecular sciences · 2025Article
- Overview of Phage Defense Systems in Bacteria and Their Applications.International journal of molecular sciences · 2024Review
- Adaptive immunity of type VI CRISPR-Cas systems associated with reverse transcriptase-Cas1 fusion proteins.Nucleic acids research · 2024Article
- Structures, mechanisms and applications of RNA-centric CRISPR-Cas13.Nature chemical biology · 2024Review
- Bioinformatic survey of CRISPR loci across 15 Serratia species.MicrobiologyOpen · 2023Article
- Review
- CRISPR-Cas systems target endogenous genes to impact bacterial physiology and alter mammalian immune responses.Molecular biomedicine · 2022Review
- Functional Features and Current Applications of the RNA-Targeting Type VI CRISPR-Cas Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.Critical reviews in biochemistry and molecular biology · 2021Review
- Evolutionary and functional classification of the CARF domain superfamily, key sensors in prokaryotic antivirus defense.Nucleic acids research · 2020Article
- Diverse enzymatic activities mediate antiviral immunity in prokaryotes.Science (New York, N.Y.) · 2020Article
- The Clustered Regularly Interspaced Short Palindromic Repeat System and Argonaute: An Emerging Bacterial Immunity System for Defense Against Natural Transformation?Frontiers in microbiology · 2020Review
- Divergent methylation of CRISPR repeats and cas genes in a subtype I-D CRISPR-Cas-system.BMC microbiology · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We show that Csx27 belongs to an expansive family of proteins that contain four predicted transmembrane helices and are typically encoded in predicted operons with components of the bacterial natural transformation machinery, multidomain proteins that consist of components of the ubiquitin signaling system and proteins containing the ligand-binding WYL domain and a helix-turn-helix domain. The Csx27 family proteins are predicted to form membrane channels for ssDNA that might comprise the core of a putative novel, Ub-regulated system for DNA uptake and, possibly, degradation. In addition to these associations, a distinct subfamily of the Csx27 family appears to be a part of a novel, membrane-associated system for DNA modification. In Bacteroidetes, subtype VI-B1 systems might degrade nascent transcripts of foreign DNA in conjunction with its uptake by the bacterial cell. These predictions suggest several experimental directions for the study of type VI CRISPR-Cas systems and distinct mechanisms of foreign DNA uptake and degradation in bacteria.
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