Evidence map›Paper›PMID 31089414›Full record

ArticleOxidative medicine and cellular longevity2019

Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes.

Matylda B Mielcarska, Magdalena Bossowska-Nowicka, Karolina P Gregorczyk-Zboroch, Zbigniew Wyżewski, Lidia Szulc-Dąbrowska, Małgorzata Gieryńska, Felix N Toka

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oxidative medicine and cellular longevity, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Matylda B MielcarskaDivision of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.ORCID 0000-0001-8302-243X
Magdalena Bossowska-NowickaDivision of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.
Karolina P Gregorczyk-ZborochDivision of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.
Zbigniew WyżewskiDepartment of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.ORCID 0000-0002-6837-0476
Lidia Szulc-DąbrowskaDivision of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.
Małgorzata GieryńskaDivision of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.ORCID 0000-0002-7865-400X
Felix N TokaDivision of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.ORCID 0000-0001-9560-8665
Warsaw University of Life Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toll-like receptors (TLRs) sense the presence of pathogen-associated molecular patterns. Nevertheless, the mechanisms modulating TLR-triggered innate immune responses are not yet fully understood. Complex regulatory systems exist to appropriately direct immune responses against foreign or self-nucleic acids, and a critical role of hepatocyte growth factor-regulated tyrosine kinase substrate (HRS), endosomal sorting complex required for transportation-0 (ESCRT-0) subunit, has recently been implicated in the endolysosomal transportation of TLR7 and TLR9. We investigated the involvement of Syk, Hrs, and STAM in the regulation of the TLR3 signaling pathway in a murine astrocyte cell line C8-D1A following cell stimulation with a viral dsRNA mimetic. Our data uncover a relationship between TLR3 and ESCRT-0, point out Syk as dsRNA-activated kinase, and suggest the role for Syk in mediating TLR3 signaling in murine astrocytes. We show molecular events that occur shortly after dsRNA stimulation of astrocytes and result in Syk Tyr-342 phosphorylation. Further, TLR3 undergoes proteolytic processing; the resulting TLR3 N-terminal form interacts with Hrs. The knockdown of Syk and Hrs enhances TLR3-mediated antiviral response in the form of IFN-

Indexed as

Adaptor Proteins, Signal TransducingAnimalsAntiviral AgentsAstrocytesCell LineEndosomal Sorting Complexes Required for TransportEnzyme ActivationInterferon Regulatory Factor-3Interferon Regulatory Factor-7LigandsMiceNF-kappa BPhosphoproteinsPhosphorylationPhosphotyrosinePoly I-CAdaptor Proteins, Signal TransducingAntiviral AgentsEndosomal Sorting Complexes Required for Transporthepatocyte growth factor-regulated tyrosine kinase substrateInterferon Regulatory Factor-3Interferon Regulatory Factor-7Irf3 protein, mouseIrf7 protein, mouseLigandsNF-kappa BPhosphoproteinsPhosphotyrosinePoly I-CStam protein, mouseSyk KinaseSyk protein, mouseTLR3 protein, mouseToll-Like Receptor 3

Identifiers

PMID31089414
PMCPMC6476135
OpenAlexW2935369448

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.