ArticleNeural regeneration research2019
RNA sequencing screening of differentially expressed genes after spinal cord injury.
Article in Neural regeneration research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Integrated multi-omics profiling uncovers miRNA-guided regulatory networks after spinal cord injury in rats.Molecular therapy. Nucleic acids · 2025Article
- Dual role of complement in neuronal repair.Frontiers in immunology · 2025Review
- ATF3 is a neuron-specific biomarker for spinal cord injury and ischaemic stroke.Clinical and translational medicine · 2024Article
- Transcriptome profile analysis in spinal cord injury rats with transplantation of menstrual blood-derived stem cells.Frontiers in molecular neuroscience · 2024Article
- Mass cytometric analysis of the immune cell landscape after traumatic brain injury elucidates the role of complement and complement receptors in neurologic outcomes.Acta neuropathologica communications · 2023Article
- RNA Sequencing and Spatial Transcriptomics in Traumatic Spinal Cord Injury (Review).Sovremennye tekhnologii v meditsine · 2023Review
- The Stress Response of the Holothurian Central Nervous System: A Transcriptomic Analysis.International journal of molecular sciences · 2022Article
- Genetic dissection of glutathione S-transferase omega-1: identification of novel downstream targets and Alzheimer's disease pathways.Neural regeneration research · 2022Article
- Analysis of gene expression profiles in two spinal cord injury models.European journal of medical research · 2022Article
- Article
- Downregulation of Nck1 After Spinal Cord Injury in Adult RatsBalkan medical journal · 2022Article
- Molecular diagnostics in neurotrauma: Are there reliable biomarkers and effective methods for their detection?Frontiers in molecular biosciences · 2022Review
- Transcriptome analysis of molecular mechanisms underlying facial nerve injury repair in rats.Neural regeneration research · 2021Article
- A 3D Fiber-Hydrogel Based Non-Viral Gene Delivery Platform Reveals that microRNAs Promote Axon Regeneration and Enhance Functional Recovery Following Spinal Cord Injury.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Expression of long non-coding RNAs in complete transection spinal cord injury: a transcriptomic analysis.Neural regeneration research · 2020Article
- RNA interference in glial cells for nerve injury treatment.Journal of tissue engineeringReview
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the search for a therapeutic schedule for spinal cord injury, it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process. However, ad hoc selection and analysis of one or two genes cannot fully reveal the complex molecular biological mechanisms of spinal cord injury. The emergence of second-generation sequencing technology (RNA sequencing) has provided a better method. In this study, RNA sequencing technology was used to analyze differentially expressed genes at different time points after spinal cord injury in rat models established by contusion of the eighth thoracic segment. The numbers of genes that changed significantly were 944, 1362 and 1421 at 1, 4 and 7 days after spinal cord injury respectively. After gene ontology analysis and temporal expression analysis of the differentially expressed genes, C5ar1, Socs3 and CCL6 genes were then selected and identified by real-time polymerase chain reaction and western blot assay. The mRNA expression trends of C5ar1, Socs3 and CCL6 genes were consistent with the RNA sequencing results. Further verification and analysis of C5ar1 indicate that the level of protein expression of C5ar1 was consistent with its nucleic acid level after spinal cord injury. C5ar1 was mainly expressed in neurons and astrocytes. Finally, the gene Itgb2, which may be related to C5ar1, was found by Chilibot database and literature search. Immunofluorescence histochemical results showed that the expression of Itgb2 was highly consistent with that of C5ar1. Itgb2 was expressed in astrocytes. RNA sequencing technology can screen differentially expressed genes at different time points after spinal cord injury. Through analysis and verification, genes strongly associated with spinal cord injury can be screened. This can provide experimental data for further determining the molecular mechanism of spinal cord injury, and also provide possible targets for the treatment of spinal cord injury. This study was approved ethically by the Laboratory Animal Ethics Committee of Jiangsu Province, China (approval No. 2018-0306-001) on March 6, 2018.
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