ArticleBMC complementary and alternative medicine2019
Bacillus/Trapa japonica Fruit Extract Ferment Filtrate enhances human hair follicle dermal papilla cell proliferation via the Akt/ERK/GSK-3β signaling pathway.
Article in BMC complementary and alternative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Multi-targeted synergy of Bacillus sp. TC5 fermentation products for transdermal drug delivery and hair regeneration.Journal of advanced research · 2026Article
- Anti-Androgenic Potential of Plant Extracts: Molecular Mechanisms, Synergistic Effects, and Nutritional Interventions.Molecules (Basel, Switzerland) · 2026Review
- 3-Hydroxypropionic Acid Enhances Hair Growth-Related Signaling in Human Follicle Dermal Papilla Cells via Activation of the Wnt/β-Catenin Pathway.International journal of molecular sciences · 2026Article
- Hair Longevity-Evidence for a Multifactorial Holistic Approach to Managing Hair Aging Changes.Journal of clinical medicine · 2025Review
- Therapeutic Potential Effect of Glycogen Synthase Kinase 3 Beta (GSK-3β) Inhibitors in Parkinson Disease: Exploring an Overlooked Avenue.Molecular neurobiology · 2024Review
- Biological Activity of Fermented Plant Extracts for Potential Dermal Applications.Pharmaceutics · 2023Review
- Identification of Antioxidative Hydrolyzable Tannins in Water Chestnut.Molecules (Basel, Switzerland) · 2023Article
- Potential Natural Products Regulation of Molecular Signaling Pathway in Dermal Papilla Stem Cells.Molecules (Basel, Switzerland) · 2023Review
- Article
- Optimizing the Fermentation Conditions ofEvidence-based complementary and alternative medicine : eCAM · 2023Article
- Effects of Baicalin on Alopecia and the Associated Mechanism.BioMed research international · 2022Article
- Antiaging Potential of Peptides from Underused Marine Bioresources.Marine drugs · 2021Review
- Article
- Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway.International journal of molecular sciences · 2020Article
- Antiaging Activity of Peptide Identified from FermentedEvidence-based complementary and alternative medicine : eCAM · 2020Article
- The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression.Scientific reports · 2019Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, creating a need for new treatments to control hair loss and prevent balding. Treatments based on plant-derived compounds could potentially prevent hair loss. Human hair follicle dermal papilla (HDP) cells, a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions such as androgenic alopecia. We examined the effect of Bacillus/Trapa japonica fruit ferment filtrate extracts (TJFs) on HDP cells to determine whether activation of the Akt/ERK/GSK-3β signaling pathway improved HDP cell proliferation.
methodsWe prepared TJFs using various methods. The extract properties were analyzed using WST-1, Lowry, and cell migration assays as well as immunofluorescence staining. We also determined the cell cycle stage and performed western blotting and an in ovo chick chorioallantoic membrane assay. Last, we constructed an organotypic three-dimensional cell culture model for immunohistochemical use.
resultsOur study confirmed that the TJFs contained numerous peptides and five unknown fractions. The TJFs stimulated HDP cell proliferation and migration via the Akt/ERK/GSK-3β signaling pathway. To verify that the Akt/ERK/GSK-3β pathway affected HDP cell proliferation, we treated HDP cells with LY294002 (an Akt inhibitor), BIO (a GSK-3β inhibitor), and PD98059 (an ERK inhibitor). The TJFs also induced cell cycle progression, inhibited type І 5α-reductase, decreased apoptosis, and enhanced angiogenesis (vascular expansion). In addition to these signaling pathways, proteins including insulin-like growth factor-1 and keratinocyte growth factor, stimulating hair growth, were detected in the three-dimensional cell culture model.
conclusionsOur results confirmed that TJFs enhance HDP cell proliferation via the Akt/ERK/GSK-3β signaling pathway, suggesting a potential treatment for alopecia.
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