ArticleParasites & vectors2019
A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review of Anopheles abundance and meta-analysis of nonhuman primate malaria infection in mosquitoes in Thailand.Parasites & vectors · 2026Pooled it
- Changes in malaria vector bionomics in Timor Leste between 2009 and 2016; a longitudinal study.Malaria journal · 2026Article
- A review and updated checklist of the Anopheles mosquitoes (Diptera: Culicidae: Anophelinae) of Thailand.Journal of medical entomology · 2026Review
- Linking Anopheles bionomics and human behaviour in the Lao PDR.Malaria journal · 2025Article
- Investigation of malaria vectors Anopheles in non-endemic areas of Thailand: in proximity to workplaces housing foreign migrant workers.Malaria journal · 2025Article
- Article
- Dirus complex species identification PCR (DiCSIP) improves the identification of Anopheles dirus complex from the Greater Mekong Subregion.Parasites & vectors · 2024Article
- Using capillary electrophoresis to identify Anopheline species in routine sampling sites.Ecology and evolution · 2024Article
- Composition of Anopheles species and bionomic characteristics over the peak malaria transmission season in Bandarban, Bangladesh.Malaria journal · 2023Article
- Myzomyia and Pyretophorus series of Anopheles mosquitoes acting as probable vectors of the goat malaria parasite Plasmodium caprae in Thailand.Scientific reports · 2023Article
- Distribution of mosquitoes (Diptera: Culicidae) in Thailand: a dataset.GigaByte (Hong Kong, China) · 2023Article
- Geometric morphometric and molecular techniques for discriminating among three cryptic species of theHeliyon · 2022Article
- Geometric morphometrics versus DNA barcoding for the identification of malaria vectors Anopheles dirus and An. baimaii in the Thai-Cambodia border.Scientific reports · 2022Article
- Community structure and insecticide resistance of malaria vectors in northern-central Myanmar.Parasites & vectors · 2022Article
- Myzorhynchus series of Anopheles mosquitoes as potential vectors of Plasmodium bubalis in Thailand.Scientific reports · 2022Article
- Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia-Laos border.Parasites & vectors · 2022Article
- An inventory of human night-biting mosquitoes and their bionomics in Sumba, Indonesia.PLoS neglected tropical diseases · 2022Article
- Redesigning Primer of ITS2 (Internal Transcribed Spacer 2) for Specific Molecular Characterization of Malaria Vectors Anopheles Species.Medical archives (Sarajevo, Bosnia and Herzegovina) · 2021Article
- Mosquitoes (Diptera: Culicidae) from Villages and Forest Areas of Rural Communes in Khanh Hoa and Binh Phuoc Provinces, Vietnam.Journal of medical entomology · 2021Article
- A multiplex PCR assay for six Aedini species, including Aedes albopictus.Parasites & vectors · 2021Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents.
resultsFor differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens.
conclusionsThis multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.
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