Evidence map›Paper›PMID 31088458›Full record

ReviewMicrobial cell factories2019

In vivo continuous evolution of metabolic pathways for chemical production.

Zheng Lin Tan, Xiang Zheng, Yinan Wu, Xingjin Jian, Xinhui Xing, Chong Zhang

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Production of ectoine by Vreelandella boliviensis using non-aseptic repeated-batch and continuous cultivations in an air-lift bioreactor.International microbiology : the official journal of the Spanish Society for Microbiology · 2025
    Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Research progress of pathway and genome evolution in microbes.Synthetic and systems biotechnology · 2022
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Automated Continuous Evolution of ProteinsACS synthetic biology · 2020
    Article
  13. Repetitive Fed-Batch: A Promising Process Mode for Biomanufacturing WithFrontiers in bioengineering and biotechnology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zheng Lin TanMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.ORCID http://orcid.org/0000-0001-6447-3788
Xiang ZhengMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Yinan WuMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Xingjin JianMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Xinhui XingMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Chong ZhangMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China. chongzhang@tsinghua.edu.cn.ORCID http://orcid.org/0000-0001-9609-8855

Funding

General Program of NSFC 21676156National Key Research and Development Program of China 2016YFF0202303National Key Scientific Instrument and Equipment Project of NSFC 21627812Tsinghua University Initiative Scientific Research Program 20161080108
6 · The paper itself

Abstract

Microorganisms have long been used as chemical plant to convert simple substrates into complex molecules. Various metabolic pathways have been optimised over the past few decades, but the progresses were limited due to our finite knowledge on metabolism. Evolution is a knowledge-free genetic randomisation approach, employed to improve the chemical production in microbial cell factories. However, evolution of large, complex pathway was a great challenge. The invention of continuous culturing systems and in vivo genetic diversification technologies have changed the way how laboratory evolution is conducted, render optimisation of large, complex pathway possible. In vivo genetic diversification, phenotypic selection, and continuous cultivation are the key elements in in vivo continuous evolution, where any human intervention in the process is prohibited. This approach is crucial in highly efficient evolution strategy of metabolic pathway evolution.

Indexed as

FermentationIndustrial MicrobiologyMetabolic EngineeringMetabolic Networks and PathwaysOrganisms, Genetically ModifiedSecondary MetabolismChemical bioproductionEquipment maintaining continuous cultureFitness-coupled selection pressureIn vivo continuous evolutionIn vivo genotype diversificationMetabolic pathway evolutionMicrobial cell factory evolution

Identifiers

PMID31088458
PMCPMC6518619

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.