Evidence map›Paper›PMID 31088324›Full record

ArticleBioengineered2019

Construction and preliminary identification of a prokaryotic expression single-chain antibody fragments library against Streptococcus pneumoniae from antibody-producing cells in human tonsil.

Jiaqi Luo, Lu Yang, Zhen Long, Zhiwen Xiao, Xiaomei Sun, Shimin Zhuang, Liangen Xie, Wei Wang, Guanping Zhang, Yue Qu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Jiaqi Luoa Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Lu Yanga Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Zhen Longa Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Zhiwen Xiaoa Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Xiaomei Suna Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Shimin Zhuanga Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Liangen Xiea Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Wei Wanga Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Guanping Zhanga Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Yue Qub Department of Pediatrics , University of Texas Medical Branch at Galveston , Guangzhou , People's Republic of China.
Tianrun Liua Departments of Otolaryngology-Head & Neck Surgery , The Sixth Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.
Sixth Affiliated Hospital of Sun Yat-sen University · CNSun Yat-sen University · CNThe University of Texas Medical Branch at Galveston · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tonsillitis is the inflammation of the tonsils due to infection, many patients ultimately have to undergo tonsillectomy. In order to improve the accuracy of diagnosis and even create a new treatment for tonsillitis, we constructed a prokaryotic expression single-chain antibody fragment library against Streptococcus pneumoniae with immunoglobulin heavy chain variable region (VH), κ light chain (Vκ), and λ light chain (Vλ) genes by using human tonsil tissue. Plasmid DNA sequencing showed that single-chain antibodies were complete and constructed correctly. The binding activity of recombinant clones was detected by enzyme-linked immunosorbent assay (ELISA), results showed that the binding activity and specificity of anti-S. pneumoniae single-chain fragment variable (scfv) is proved to be successful. The single-chain antibody may be an attractive strategy for tonsillitis etiologic diagnosis and therapy.

Indexed as

Enzyme-Linked Immunosorbent AssayHumansPalatine TonsilPlasmidsSingle-Chain AntibodiesStreptococcus pneumoniaeSingle-Chain Antibodiesantibody librarychronic tonsillitisPneumococcal infectionssingle-chain fragment variable (scfv)

Identifiers

PMID31088324
PMCPMC6527077
OpenAlexW2944894068

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.